The Proliferation of Cervical Cancer is Promoted by MiRNA-125b Through the Regulation of the HMGA1

Overview

Journal Onco Targets Ther

Publisher Dove Medical Press

Specialty Oncology

Date 2019 May 3

PMID 31043793

Citations 14

Authors

Bingmei Sun

Ying Zhang

Lianxiang Zhou

Linin Yin

Fei Li

Chao Li

Jiayu Xia

Affiliations

Soon will be listed here.

Abstract

Background: It has been reported that miRNA-125b is associated with carcinogenesis and development of several different kinds of cancers. Nevertheless, there is no clarity regarding the significance and mechanism of action of miR-125b in clinical practice for cervical cancer (CC).

Materials And Methods: In the current investigation, the expression of miR-125b in cervical clinical specimens and CC cell lines was analyzed via real-time quantitative PCR, and the relationship of miR-125b with the chromatin-associated protein high mobility group A (HMGA1) expression and clinicopathological parameters of CC patients was explored.

Results: The results indicated that miR-125b expression was remarkably upregulated in CC cell lines as well as in the tissues of humans. miR-125b overexpression was significantly related to a decrease in HMGA1 expression, progression-free survival, overall survival, and prognosis as well. Besides, knockdown of miR-125b inhibited proliferation and colony formation in SW756 and C4-1 cells, where the 3'-UTR of HMGA1 mRNA was directly targeted. Moreover, PI3K/Akt pathway was regulated by miR-125b through suppression of HMGA1.

Conclusion: These findings illustrated that a new regulatory role of HMGA1 is involved in the progression of CC. Our data demonstrated that miR-125b could play a critical role in the carcinogenesis and progression of CC, revealing that miR-125b might serve as a potential new target for treating CC.

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References

Lee Y, Dutta A . MicroRNAs in cancer. Annu Rev Pathol. 2008; 4:199-227. PMC: 2769253. DOI: 10.1146/annurev.pathol.4.110807.092222. View

Xi Y, Li Y, Tang H . High mobility group A1 protein acts as a new target of Notch1 signaling and regulates cell proliferation in T leukemia cells. Mol Cell Biochem. 2012; 374(1-2):173-80. DOI: 10.1007/s11010-012-1517-2. View

Dizon D, MacKay H, Thomas G, Werner T, Kohn E, Hess D . State of the science in cervical cancer: where we are today and where we need to go. Cancer. 2014; 120(15):2282-8. DOI: 10.1002/cncr.28722. View

Shin V, Chu K . MiRNA as potential biomarkers and therapeutic targets for gastric cancer. World J Gastroenterol. 2014; 20(30):10432-9. PMC: 4130850. DOI: 10.3748/wjg.v20.i30.10432. View

Wu J, Wang J, Jiang X, Lan J, He X, Wang H . MiR-125b promotes cell migration and invasion by targeting PPP1CA-Rb signal pathways in gastric cancer, resulting in a poor prognosis. Gastric Cancer. 2014; 18(4):729-39. DOI: 10.1007/s10120-014-0421-8. View

Wang W, Chen Y . Circulating miRNAs in cancer: from detection to therapy. J Hematol Oncol. 2014; 7:86. PMC: 4269921. DOI: 10.1186/s13045-014-0086-0. View

Li X, Zheng J, Chen L, Diao H, Liu Y . Predictive and Prognostic Roles of Abnormal Expression of Tissue miR-125b, miR-221, and miR-222 in Glioma. Mol Neurobiol. 2014; 53(1):577-583. DOI: 10.1007/s12035-014-9017-x. View

He Y, Lin J, Ding Y, Liu G, Luo Y, Huang M . A systematic study on dysregulated microRNAs in cervical cancer development. Int J Cancer. 2015; 138(6):1312-27. DOI: 10.1002/ijc.29618. View

Zhang Y, Zhang D, Wang F, Xu D, Guo Y, Cui W . Serum miRNAs panel (miR-16-2*, miR-195, miR-2861, miR-497) as novel non-invasive biomarkers for detection of cervical cancer. Sci Rep. 2015; 5:17942. PMC: 4677300. DOI: 10.1038/srep17942. View

10.

Alldredge J, Tewari K . Clinical Trials of Antiangiogenesis Therapy in Recurrent/Persistent and Metastatic Cervical Cancer. Oncologist. 2016; 21(5):576-85. PMC: 4861362. DOI: 10.1634/theoncologist.2015-0393. View

11.

Zhou Q, Han L, Zhou Y, Li Y . MiR-195 Suppresses Cervical Cancer Migration and Invasion Through Targeting Smad3. Int J Gynecol Cancer. 2016; 26(5):817-24. DOI: 10.1097/IGC.0000000000000686. View

12.

Bu Q, You F, Pan G, Yuan Q, Cui T, Hao L . MiR-125b inhibits anaplastic thyroid cancer cell migration and invasion by targeting PIK3CD. Biomed Pharmacother. 2017; 88:443-448. DOI: 10.1016/j.biopha.2016.11.090. View

13.

Cheng H, Chen Y, Wong J, Weng C, Chen H, Yu S . Cancer cells increase endothelial cell tube formation and survival by activating the PI3K/Akt signalling pathway. J Exp Clin Cancer Res. 2017; 36(1):27. PMC: 5296960. DOI: 10.1186/s13046-017-0495-3. View

14.

. Cervical Cancer Analysis Reveals New Mutations. Cancer Discov. 2017; 7(4):344. DOI: 10.1158/2159-8290.CD-NB2017-020. View

15.

Granados-Lopez A, Ruiz-Carrillo J, Servin-Gonzalez L, Martinez-Rodriguez J, Reyes-Estrada C, Gutierrez-Hernandez R . Use of Mature miRNA Strand Selection in miRNAs Families in Cervical Cancer Development. Int J Mol Sci. 2017; 18(2). PMC: 5343941. DOI: 10.3390/ijms18020407. View

16.

Bahrami A, Hasanzadeh M, Hassanian S, Shahidsales S, Ghayour-Mobarhan M, Ferns G . The Potential Value of the PI3K/Akt/mTOR Signaling Pathway for Assessing Prognosis in Cervical Cancer and as a Target for Therapy. J Cell Biochem. 2017; 118(12):4163-4169. DOI: 10.1002/jcb.26118. View

17.

Liu W, Hu J, Zhou K, Chen F, Wang Z, Liao B . Serum exosomal miR-125b is a novel prognostic marker for hepatocellular carcinoma. Onco Targets Ther. 2017; 10:3843-3851. PMC: 5546809. DOI: 10.2147/OTT.S140062. View

18.

Liang H, Luo R, Chen X, Zhao Y, Tan A . miR-187 inhibits the growth of cervical cancer cells by targeting FGF9. Oncol Rep. 2017; 38(4):1977-1984. PMC: 5652944. DOI: 10.3892/or.2017.5916. View

19.

Tanaka H, Hazama S, Iida M, Tsunedomi R, Takenouchi H, Nakajima M . miR-125b-1 and miR-378a are predictive biomarkers for the efficacy of vaccine treatment against colorectal cancer. Cancer Sci. 2017; 108(11):2229-2238. PMC: 5666028. DOI: 10.1111/cas.13390. View

20.

Yang L, Zhang X, Ma Y, Zhao X, Li B, Wang H . Ascites promotes cell migration through the repression of miR-125b in ovarian cancer. Oncotarget. 2017; 8(31):51008-51015. PMC: 5584225. DOI: 10.18632/oncotarget.16846. View