» Articles » PMID: 31041723

Analysis of TRNA Processing in the Absence of CCAase in Bacillus Subtilis

Overview
Specialty Microbiology
Date 2019 May 2
PMID 31041723
Citations 2
Authors
Affiliations
Soon will be listed here.
Abstract

In Bacillus subtilis, the tRNA lacks an encoded CCA 3' end. To gain insight into the role of CCAase and RNases in tRNA processing, several mutant strains were generated. Northern blot and RT-PCR results suggest that enzymes other than CCAase can participate in CCA addition at the 3' end of the immature tRNA.

Citing Articles

A model industrial workhorse: Bacillus subtilis strain 168 and its genome after a quarter of a century.

Bremer E, Calteau A, Danchin A, Harwood C, Helmann J, Medigue C Microb Biotechnol. 2023; 16(6):1203-1231.

PMID: 37002859 PMC: 10221552. DOI: 10.1111/1751-7915.14257.


Analysis of tRNA processing under salt stress in spore outgrowth using RNA sequencing data.

Arvizu Hernandez I, Flores J, Caballero Perez J, Gutierrez Sanchez H, Lopez M, Gomez S F1000Res. 2021; 9:501.

PMID: 33976872 PMC: 8097732. DOI: 10.12688/f1000research.23780.1.

References
1.
Wen T, Oussenko I, Pellegrini O, Bechhofer D, Condon C . Ribonuclease PH plays a major role in the exonucleolytic maturation of CCA-containing tRNA precursors in Bacillus subtilis. Nucleic Acids Res. 2005; 33(11):3636-43. PMC: 1160522. DOI: 10.1093/nar/gki675. View

2.
Reuven N, Zhou Z, Deutscher M . Functional overlap of tRNA nucleotidyltransferase, poly(A) polymerase I, and polynucleotide phosphorylase. J Biol Chem. 1998; 272(52):33255-9. DOI: 10.1074/jbc.272.52.33255. View

3.
Campos-Guillen J, Bralley P, Jones G, Bechhofer D, Olmedo-Alvarez G . Addition of poly(A) and heteropolymeric 3' ends in Bacillus subtilis wild-type and polynucleotide phosphorylase-deficient strains. J Bacteriol. 2005; 187(14):4698-706. PMC: 1169516. DOI: 10.1128/JB.187.14.4698-4706.2005. View

4.
Oussenko I, Abe T, Ujiie H, Muto A, Bechhofer D . Participation of 3'-to-5' exoribonucleases in the turnover of Bacillus subtilis mRNA. J Bacteriol. 2005; 187(8):2758-67. PMC: 1070398. DOI: 10.1128/JB.187.8.2758-2767.2005. View

5.
Vold B . Structure and organization of genes for transfer ribonucleic acid in Bacillus subtilis. Microbiol Rev. 1985; 49(1):71-80. PMC: 373018. DOI: 10.1128/mr.49.1.71-80.1985. View