New UHPLC-QqQ-MS/MS Method for the Rapid and Sensitive Analysis of Ascorbic and Dehydroascorbic Acids in Plant Foods

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2019 Apr 28

PMID 31027237

Citations 8

Authors

Nieves Baenas

Francisco J Salar

Raul Dominguez-Perles

Cristina Garcia-Viguera

Affiliations

Soon will be listed here.

Abstract

A new method using ultra high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UHPLC-QqQ-MS/MS) methodology was developed for the determination of ascorbic acid (AA) and dehydroascorbic acid (DHAA) contents in liquid and solid vegetable samples. The advantages of this method are speed, high sensitivity and practical application. In accordance with these advantages, the present method allows the simultaneous determination of AA and DHAA without previous reduction/derivatization of DHAA and without the use of internal standards in the samples. This is of high interest in routine analysis, providing a simpler sample preparation, as well as enhanced accuracy and robustness. Its validation included selectivity, sensitivity and linearity, precision and accuracy, matrix effect, and recovery. The results showed high selectivity and sensitivity, with calibration curves ranging from 10 to 500 ng mL and from 50 to 500 ng mL for AA and DHAA, respectively. Appropriate dilutions for each sample are necessary to avoid the matrix effect with accepted recoveries.

Citing Articles

Dehydroascorbic acid quantification in human plasma: Simultaneous direct measurement of the ascorbic acid/dehydroascorbic acid couple by UPLC/MS-MS.

Violet P, Munyan N, Luecke H, Wang Y, Lloyd J, Patra K Redox Biol. 2024; 78:103425.

PMID: 39591903 PMC: 11626825. DOI: 10.1016/j.redox.2024.103425.

Microwave Treatment vs. Conventional Pasteurization: The Effect on Phytochemical and Microbiological Quality for Citrus-Maqui Beverages.

Salar F, Diaz-Morcillo A, Fayos-Fernandez J, Monzo-Cabrera J, Sanchez-Bravo P, Dominguez-Perles R Foods. 2024; 13(1).

PMID: 38201129 PMC: 10778303. DOI: 10.3390/foods13010101.

CeO-CoO nanocomposite with oxidase-like activity for colorimetric detection of ascorbic acid.

Yang J, Cheng S, Qin S, Huang L, Xu Y, Wang Y RSC Adv. 2023; 13(15):9918-9923.

PMID: 36998518 PMC: 10043987. DOI: 10.1039/d3ra01074k.

Fusion of 2DGC-MS, HPLC-MS and Sensory Data to Assist Decision-Making in the Marketing of International Monovarietal Chardonnay and Sauvignon Blanc Wines.

Poggesi S, Darnal A, Ceci A, Longo E, Vanzo L, Mimmo T Foods. 2022; 11(21).

PMID: 36360071 PMC: 9657765. DOI: 10.3390/foods11213458.

Influence of Sweeteners (Sucrose, Sucralose, and Stevia) on Bioactive Compounds in a Model System Study for Citrus-Maqui Beverages.

Salar F, Agullo V, Dominguez-Perles R, Garcia-Viguera C Foods. 2022; 11(15).

PMID: 35954034 PMC: 9368535. DOI: 10.3390/foods11152266.

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