Methamphetamine-induced Apoptosis in Glial Cells Examined Under Marker-free Imaging Modalities

Overview

Journal J Biomed Opt

Specialties Biomedical Engineering
Ophthalmology

Date 2019 Apr 27

PMID 31025559

Citations 6

Authors

Lianna Y DBrant

Habben Desta

Ting Chean Khoo

Anna V Sharikova

Supriya D Mahajan

Alexander Khmaladze

Affiliations

Soon will be listed here.

Abstract

We used phase microscopy and Raman spectroscopic measurements to assess the response of in vitro rat C6 glial cells following methamphetamine treatment in real time. Digital holographic microscopy (DHM) and three-dimensional (3-D) tomographic nanoscopy allow measurements of live cell cultures, which yield information about cell volume changes. Tomographic phase imaging provides 3-D information about the refractive index distribution associated with the morphology of biological samples. DHM provides similar information, but for a larger population of cells. Morphological changes in cells are associated with alterations in cell cycle and initiation of cell death mechanisms. Raman spectroscopy measurements provide information about chemical changes within the cells. Our Raman data indicate that the chemical changes in proteins preceded morphological changes, which were seen with DHM. Our study also emphasizes that tomographic phase imaging, DHM, and Raman spectroscopy are imaging tools that can be utilized for noninvasive simultaneous monitoring of morphological and chemical changes in cells during apoptosis and can also be used to monitor other dynamic cell processes.

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References

Salamanca S, Sorrentino E, Nosanchuk J, Martinez L . Impact of methamphetamine on infection and immunity. Front Neurosci. 2015; 8:445. PMC: 4290678. DOI: 10.3389/fnins.2014.00445. View

Sadeghi-Aliabadi H, Minaiyan M, Dabestan A . Cytotoxic evaluation of doxorubicin in combination with simvastatin against human cancer cells. Res Pharm Sci. 2011; 5(2):127-33. PMC: 3203264. View

Basova L, Najera J, Bortell N, Wang D, Moya R, Lindsey A . Dopamine and its receptors play a role in the modulation of CCR5 expression in innate immune cells following exposure to Methamphetamine: Implications to HIV infection. PLoS One. 2018; 13(6):e0199861. PMC: 6019408. DOI: 10.1371/journal.pone.0199861. View

Zhu J, Xu W, Angulo J . Methamphetamine-induced cell death: selective vulnerability in neuronal subpopulations of the striatum in mice. Neuroscience. 2006; 140(2):607-22. PMC: 2882192. DOI: 10.1016/j.neuroscience.2006.02.055. View

Khmaladze A, Kim M, Lo C . Phase imaging of cells by simultaneous dual-wavelength reflection digital holography. Opt Express. 2008; 16(15):10900-11. DOI: 10.1364/oe.16.010900. View

Parekh R, Ascoli G . Neuronal morphology goes digital: a research hub for cellular and system neuroscience. Neuron. 2013; 77(6):1017-38. PMC: 3653619. DOI: 10.1016/j.neuron.2013.03.008. View

McElderry J, Kole M, Morris M . Repeated freeze-thawing of bone tissue affects Raman bone quality measurements. J Biomed Opt. 2011; 16(7):071407. PMC: 3144971. DOI: 10.1117/1.3574525. View

Khmaladze A, Kuo S, Kim R, Matthews R, Marcelo C, Feinberg S . Human oral mucosa tissue-engineered constructs monitored by Raman fiber-optic probe. Tissue Eng Part C Methods. 2014; 21(1):46-51. PMC: 4291158. DOI: 10.1089/ten.TEC.2013.0622. View

Boss D, Kuhn J, Jourdain P, Depeursinge C, Magistretti P, Marquet P . Measurement of absolute cell volume, osmotic membrane water permeability, and refractive index of transmembrane water and solute flux by digital holographic microscopy. J Biomed Opt. 2013; 18(3):036007. DOI: 10.1117/1.JBO.18.3.036007. View

10.

Tsutsui-Kimura I, Takiue H, Yoshida K, Xu M, Yano R, Ohta H . Dysfunction of ventrolateral striatal dopamine receptor type 2-expressing medium spiny neurons impairs instrumental motivation. Nat Commun. 2017; 8:14304. PMC: 5296642. DOI: 10.1038/ncomms14304. View

11.

Kasprowicz R, Suman R, OToole P . Characterising live cell behaviour: Traditional label-free and quantitative phase imaging approaches. Int J Biochem Cell Biol. 2017; 84:89-95. DOI: 10.1016/j.biocel.2017.01.004. View

12.

Rappaz B, Breton B, Shaffer E, Turcatti G . Digital holographic microscopy: a quantitative label-free microscopy technique for phenotypic screening. Comb Chem High Throughput Screen. 2013; 17(1):80-8. PMC: 3894694. DOI: 10.2174/13862073113166660062. View

13.

Griffiths G, Lucocq J . Antibodies for immunolabeling by light and electron microscopy: not for the faint hearted. Histochem Cell Biol. 2014; 142(4):347-60. PMC: 4160575. DOI: 10.1007/s00418-014-1263-5. View

14.

Aydinlik S, Erkisa M, Cevatemre B, Sarimahmut M, Dere E, Ari F . Enhanced cytotoxic activity of doxorubicin through the inhibition of autophagy in triple negative breast cancer cell line. Biochim Biophys Acta Gen Subj. 2016; 1861(2):49-57. DOI: 10.1016/j.bbagen.2016.11.013. View

15.

Deng X, Cadet J . Methamphetamine-induced apoptosis is attenuated in the striata of copper-zinc superoxide dismutase transgenic mice. Brain Res Mol Brain Res. 2000; 83(1-2):121-4. DOI: 10.1016/s0169-328x(00)00169-8. View

16.

Filali S, Geloen A, Lysenko V, Pirot F, Miossec P . Live-stream characterization of cadmium-induced cell death using visible CdTe-QDs. Sci Rep. 2018; 8(1):12614. PMC: 6105671. DOI: 10.1038/s41598-018-31077-2. View

17.

Elmore S . Apoptosis: a review of programmed cell death. Toxicol Pathol. 2007; 35(4):495-516. PMC: 2117903. DOI: 10.1080/01926230701320337. View

18.

Marquet P, Rappaz B, Magistretti P, Cuche E, Emery Y, Colomb T . Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy. Opt Lett. 2005; 30(5):468-70. DOI: 10.1364/ol.30.000468. View

19.

Sharikova A, Quaye E, Park J, Maloney M, Desta H, Thiyagarajan R . Methamphetamine Induces Apoptosis of Microglia via the Intrinsic Mitochondrial-Dependent Pathway. J Neuroimmune Pharmacol. 2018; 13(3):396-411. DOI: 10.1007/s11481-018-9787-4. View

20.

Bergman O, Ben-Shachar D . Mitochondrial Oxidative Phosphorylation System (OXPHOS) Deficits in Schizophrenia: Possible Interactions with Cellular Processes. Can J Psychiatry. 2016; 61(8):457-69. PMC: 4959648. DOI: 10.1177/0706743716648290. View