Distinct RanBP1 Nuclear Export and Cargo Dissociation Mechanisms Between Fungi and Animals

Overview

Journal Elife

Specialty Biology

Date 2019 Apr 26

PMID 31021318

Citations 10

Authors

Yuling Li

Jinhan Zhou

Sui Min

Yang Zhang

Yuqing Zhang

Qiao Zhou

Xiaofei Shen

Da Jia

Junhong Han

Qingxiang Sun

Affiliations

Soon will be listed here.

Abstract

Ran binding protein 1 (RanBP1) is a cytoplasmic-enriched and nuclear-cytoplasmic shuttling protein, playing important roles in nuclear transport. Much of what we know about RanBP1 is learned from fungi. Intrigued by the long-standing paradox of harboring an extra NES in animal RanBP1, we discovered utterly unexpected cargo dissociation and nuclear export mechanisms for animal RanBP1. In contrast to CRM1-RanGTP sequestration mechanism of cargo dissociation in fungi, animal RanBP1 solely sequestered RanGTP from nuclear export complexes. In fungi, RanBP1, CRM1 and RanGTP formed a 1:1:1 nuclear export complex; in contrast, animal RanBP1, CRM1 and RanGTP formed a 1:1:2 nuclear export complex. The key feature for the two mechanistic changes from fungi to animals was the loss of affinity between RanBP1-RanGTP and CRM1, since residues mediating their interaction in fungi were not conserved in animals. The biological significances of these different mechanisms in fungi and animals were also studied.

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