Capacitive Sensor to Monitor Enzyme Activity by Following Degradation of Macromolecules in Real Time

Overview

Journal Appl Biochem Biotechnol

Specialties Biochemistry
Biotechnology

Date 2019 Apr 26

PMID 31020512

Authors

Gizem Erturk Bergdahl

Martin Hedstrom

Bo Mattiasson

Affiliations

Soon will be listed here.

Abstract

A capacitive sensor was developed to analyze the presence and enzymatic activity of a model protease from standard solutions by following the degradation of the substrate in real time. The enzyme was chosen based on its specific digestion of the hinge region of immunoglobulin G (IgG). Real-time enzyme activity was monitored by measuring the change in capacitance (∆C) based on the release of IgG fragments after enzymatic digestion by the enzyme. The results indicated that the developed capacitive system might be used successfully for label-free and real-time monitoring of enzymatic activity of different enzymes in a sensitive, rapid, and inexpensive manner in biotechnological, environmental, and clinical applications.

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