Polymorphic Analysis of Leptin Promoter in Obese/diabetic Subjects in Kashmiri Population

Overview

Journal Indian J Endocrinol Metab

Specialty Endocrinology

Date 2019 Apr 25

PMID 31016164

Citations 1

Authors

Rubiya Dar

Shabhat Rasool

Ajaz Ahmad Waza

Gazalla Ayoub

Meenu Qureshi

Abdul Hamid Zargar

Iftikhar Bashir

Tariq Jan

Khurshid Iqbal Andrabi

Affiliations

Soon will be listed here.

Abstract

Background: The role of common variants in leptin promoter has already been established to play a major role in obesity and diabetes in humans. The study was accordingly focused on leptin promoter variants and their potential association with diabetes and obesity in ethnic population from Kashmir, India.

Methods: Allele frequencies of 620 Kashmiri subjects with diabetes (200), obese subjects (200), and ethnically matched healthy controls (200) were tested for the Hardy-Weinberg disequilibrium. Among 200 obese subjects, a total of 50 persons were with diabetes. The genotype and allele frequencies were evaluated using the Chi-square or Fisher's exact tests.

Results: Sequence analysis revealed two reported variations i.e., rs72563764C>T and rs7799039G>A in promoter region. Both variants show homozygous as well as heterozygous genotypes. These variations indicated significant difference with respect to allelic and genotypic frequencies in all groups i.e., persons with diabetes, obese, and obese persons with diabetes ( < 0.05). We also analyzed the association of these variations with biochemical characteristics and found significant association of rs72563764C>T with triglycerides (TG) in obese patients and fasting plasma glucose (FPG) and random blood sugar (RBS) in obese/persons with diabetes. Also rs7799039G>A showed association with postprandial plasma sugar (PPPS) in obese patients and FPG and resting plasma glucose (RPG) in obese persons with diabetes.

Conclusions: Our results are suggestive of the association of leptin promoter gene variations i.e., rs72563764C>T and rs7799039G>A with both diabetes and obesity.

Citing Articles

Genetic Association of LEP Gene Polymorphisms with Obesity in Moroccan Individuals: Case-Control Study and Updated Meta-analysis.

Bouafi H, Krami A, Morjane I, Slaoui K, Harmak H, Charoute H Biochem Genet. 2023; 61(5):1758-1774.

PMID: 36792840 DOI: 10.1007/s10528-023-10342-8.

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