Mutations in a New Chromosomal Gene of Escherichia Coli K-12, PcnB, Reduce Plasmid Copy Number of PBR322 and Its Derivatives

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1986 Nov 1

PMID 3100913

Citations 136

Authors

J Lopilato

S Bortner

J Beckwith

Affiliations

Soon will be listed here.

Abstract

We describe mutants of Escherichia coli that decrease the plasmid copy number of pBR322 derivatives. One mutant was partially characterized genetically and its mutation, designated pcnB for plasmid copy number, was mapped to approximately 3 min on the E. coli chromosome. This locus is distinct from other genes whose products are known to affect plasmid replication or stable plasmid maintenance. The pcnB mutant strain should be useful for cloning genes into pBR322 that have aberrant or deleterious effects on the cell when present in high copy number.

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