MicroRNA-132-3p Represses Smad5 in MC3T3-E1 Osteoblastic Cells Under Cyclic Tensile Stress

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 2019 Apr 21

PMID 31004309

Citations 8

Authors

Mingyan Liu

Fen Sun

Yunxia Feng

Xinyi Sun

Juan Li

Qiang Fan

Ming Liu

Affiliations

Soon will be listed here.

Abstract

MicroRNAs (miRNAs) regulate osteogenic differentiation of bone cells, which has applications in orthodontics. Here we evaluated the miRNA expression profile of MC3T3-E1 osteoblasts under cyclic tensile stress with chip technology and found that miR-132-3p was up-regulated by 12% cyclic tensile stress. Alkaline phosphatase activity and osteocalcin expression in MC3T3-E1 cells were decreased under these conditions. Smad2 and Smad5 were identified as potential target genes of miR-132-3p. Native and phosphorylated Smad2 and Smad5 expression was negatively correlated with miR-132-3p levels in the cells under cyclic stretch; however, only Smad5 protein level was reduced upon miR-132-3p overexpression. The luciferase reporter assay confirmed a direct interaction between miR-132-3p and Smad5. Thus, miR-132-3p maybe regulates osteoblast differentiation via Smad5 in response to cyclic tensile stress.

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