Synaptic Vesicle Recycling Pathway Determines Neurotransmitter Content and Release Properties

Overview

Journal Neuron

Publisher Cell Press

Specialty Neurology

Date 2019 Apr 21

PMID 31003725

Citations 66

Authors

Katlin Silm

Jing Yang

Pamela F Marcott

Cedric S Asensio

Jacob Eriksen

Daryl A Guthrie

Amy H Newman

Christopher P Ford

Robert H Edwards

Affiliations

Soon will be listed here.

Abstract

In contrast to temporal coding by synaptically acting neurotransmitters such as glutamate, neuromodulators such as monoamines signal changes in firing rate. The two modes of signaling have been thought to reflect differences in release by different cells. We now find that midbrain dopamine neurons release glutamate and dopamine with different properties that reflect storage in different synaptic vesicles. The vesicles differ in release probability, coupling to presynaptic Ca channels and frequency dependence. Although previous work has attributed variation in these properties to differences in location or cytoskeletal association of synaptic vesicles, the release of different transmitters shows that intrinsic differences in vesicle identity drive different modes of release. Indeed, dopamine but not glutamate vesicles depend on the adaptor protein AP-3, revealing an unrecognized linkage between the pathway of synaptic vesicle recycling and the properties of exocytosis. Storage of the two transmitters in different vesicles enables the transmission of distinct signals.

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