Set3 Is Required for Asexual Development, Aflatoxin Biosynthesis, and Fungal Virulence in

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2019 Apr 20

PMID 31001207

Citations 9

Authors

Huahui Lan

Lianghuan Wu

Kun Fan

Ruilin Sun

Guang Yang

Feng Zhang

Kunlong Yang

Xiaolu Lin

Yanhong Chen

Jun Tian

Shihua Wang

Affiliations

Soon will be listed here.

Abstract

is an opportunistic pathogenic fungus for both plant and animal that produces carcinogenic toxins termed aflatoxins (AFs). To identify possible genetic targets to reduce AF contamination, in this study, we have characterized a novel Set3, and it shares sequence homology with the yeast protein Set3. The deletion mutants present no difference in growth rate but alterations in asexual development and secondary metabolite production when compared to the wild type. Specifically, deletion of gene decreases conidiophore formation and conidial production through downregulating expression of and genes. In addition, normal levels of are required for sclerotial development and expression of sclerotia-related genes and . Further analyses demonstrated that Set3 negatively regulates AF production as well as the concomitant expression of genes in the AF gene cluster. Importantly, our results also display that Set3 is involved in crop kernel colonization. Taking together, these results reveal that a novel Set3 plays crucial roles in morphological development, secondary metabolism, and fungal virulence in .

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