Biosynthesis of DHGA and Its Roles in Arabidopsis Seedling Establishment

Overview

Journal Nat Commun

Specialty Biology

Date 2019 Apr 18

PMID 30992454

Citations 28

Authors

Hao Liu

Siyi Guo

Minghua Lu

Yu Zhang

Junhua Li

Wei Wang

Pengtao Wang

Junli Zhang

Zhubing Hu

Liangliang Li

Lingyu Si

Jie Zhang

Qi Qi

Xiangning Jiang

Jose Ramon Botella

Hua Wang

Chun-Peng Song

Affiliations

Soon will be listed here.

Abstract

Seed germination and photoautotrophic establishment are controlled by the antagonistic activity of the phytohormones gibberellins (GAs) and abscisic acid (ABA). Here we show that Arabidopsis thaliana GAS2 (Gain of Function in ABA-modulated Seed Germination 2), a protein belonging to the Fe-dependent 2-oxoglutarate dioxygenase superfamily, catalyzes the stereospecific hydration of GA to produce GA 16, 17-dihydro-16α-ol (DHGA). We show that DHGA, a C-GA has an atypical structure compared to known active GAs but can bind to the GA receptor (GID1c). DHGA can promote seed germination, hypocotyl elongation and cotyledon greening. Silencing and over-expression of GAS2 alters the ABA/GA ratio and sensitivity to ABA during seed germination and photoautotrophic establishment. Hence, we propose that GAS2 acts to modulate hormonal balance during early seedling development.

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