Transfer of Arachidonate from Phosphatidylcholine to Phosphatidylethanolamine and Triacylglycerol in Guinea Pig Alveolar Macrophages

Overview

Journal Lipids

Specialty Biochemistry

Date 1986 Oct 1

PMID 3099114

Citations 2

Authors

J G Nijssen

R S Oosting

F P Nykamp

H van den Bosch

Affiliations

Soon will be listed here.

Abstract

Guinea pig alveolar macrophages were labeled by incubation with either arachidonate or linoleate. Arachidonate labeled phosphatidylcholine (PC), phosphatidylethanolamine (PE) and triglycerides (TG) equally well, with each lipid containing about 30% of total cellular radioactivity. In comparison to arachidonate, linoleate was recovered significantly less in PE (7%) and more in TG (47%). To investigate whether redistributions of acyl chains among lipid classes took place, the macrophages were incubated with 1-acyl-2-[1-14C]arachidonoyl PC or 1-acyl-2-[1-14C]linoleoyl PC. After harvesting, the cells incubated with 1-acyl-2-[1-14C]linoleoyl PC contained 86% of the recovered cellular radioactivity in PC, with only small amounts of label being transferred to PE and TG (3 and 6%, respectively). More extensive redistributions were observed with arachidonate-labeled PC. In this case, only 60% of cellular radioactivity was still associated with PC, while 22 and 12%, respectively, had been transferred to PE and TG. Arachidonate transfer from PC to PE was unaffected by an excess of free arachidonate which inhibited this transfer to TG for over 90%, indicating that different mechanisms or arachidonoyl CoA pools were involved in the transfer of arachidonate from PC to PE and TG. Cells prelabeled with 1-acyl-2-[1-14C]arachidonoyl PC released 14C-label into the medium upon further incubation. This release was slightly stimulated by zymosan and threefold higher in the presence of the Ca2+-ionophore A23187. Labeling of macrophages with intact phospholipid molecules appears to be a suitable method for studying acyl chain redistribution and release reactions.

Citing Articles

Uptake, incorporation and metabolism of (3H)triolein in the isolated perfused rabbit heart.

Weis M, Palazzo A, Williams Jr J, Malik K Lipids. 1990; 25(8):497-503.

PMID: 2215090 DOI: 10.1007/BF02538094.

Oxysterol mediated changes in fatty acid distribution and lipid synthesis in cultured bovine aortic smooth muscle cells.

Seillan C Lipids. 1990; 25(3):172-6.

PMID: 2110280 DOI: 10.1007/BF02544334.

References

KRAMER R, Deykin D . Arachidonoyl transacylase in human platelets. Coenzyme A-independent transfer of arachidonate from phosphatidylcholine to lysoplasmenylethanolamine. J Biol Chem. 1983; 258(22):13806-11. View

Kouzan S, Brody A, Nettesheim P, Eling T . Production of arachidonic acid metabolites by macrophages exposed in vitro to asbestos, carbonyl iron particles, or calcium ionophore. Am Rev Respir Dis. 1985; 131(4):624-32. DOI: 10.1164/arrd.1985.131.4.624. View

Albert D, Snyder F . Release of arachidonic acid from 1-alkyl-2-acyl-sn-glycero-3-phosphocholine, a precursor of platelet-activating factor, in rat alveolar macrophages. Biochim Biophys Acta. 1984; 796(1):92-101. DOI: 10.1016/0005-2760(84)90242-x. View

Trotter J, Flesch I, Schmidt B, FERBER E . Acyltransferase-catalyzed cleavage of arachidonic acid from phospholipids and transfer to lysophosphatides in lymphocytes and macrophages. J Biol Chem. 1982; 257(4):1816-23. View

KRAMER R, Pritzker C, Deykin D . Coenzyme A-mediated arachidonic acid transacylation in human platelets. J Biol Chem. 1984; 259(4):2403-6. View

Flesch I, Ecker B, FERBER E . Acyltransferase-catalyzed cleavage of arachidonic acid from phospholipids and transfer to lysophosphatides in macrophages derived from bone marrow. Comparison of different donor- and acceptor substrate combinations. Eur J Biochem. 1984; 139(3):431-7. DOI: 10.1111/j.1432-1033.1984.tb08023.x. View

Kessels J, Ousen H, van den Bosch H . Facilitated utilization of endogenously synthesized lysophosphatidic acid by 1-acylglycerophosphate acyltransferase from Escherichia coli. Biochim Biophys Acta. 1983; 753(2):227-35. DOI: 10.1016/0005-2760(83)90011-5. View

Albert D, Snyder F . Biosynthesis of 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor) from 1-alkyl-2-acyl-sn-glycero-3-phosphocholine by rat alveolar macrophages. Phospholipase A2 and acetyltransferase activities during phagocytosis and.... J Biol Chem. 1983; 258(1):97-102. View

Holub B . Specific formation of arachidonoyl phosphatidylinositol from 1-acyl-sn-glycero-3-phosphorylinositol in rat liver. Lipids. 1976; 11(1):1-5. DOI: 10.1007/BF02532576. View

10.

Peters-Golden M, Bathon J, Flores R, Hirata F, NEWCOMBE D . Glucocorticoid inhibition of zymosan-induced arachidonic acid release by rat alveolar macrophages. Am Rev Respir Dis. 1984; 130(5):803-9. DOI: 10.1164/arrd.1984.130.5.803. View

11.

van den Bosch H, Aarsman A . A review on methods of phospholipase A determination. Agents Actions. 1979; 9(4):382-9. DOI: 10.1007/BF01970666. View

12.

Robinson M, Blank M, Snyder F . Acylation of lysophospholipids by rabbit alveolar macrophages. Specificities of CoA-dependent and CoA-independent reactions. J Biol Chem. 1985; 260(13):7889-95. View

13.

Westerman J, Kamp H, Wirtz K . Phosphatidylcholine transfer protein from bovine liver. Methods Enzymol. 1983; 98:581-6. DOI: 10.1016/0076-6879(83)98185-5. View

14.

Emilsson A, Sundler R . Studies on the enzymatic pathways of calcium ionophore-induced phospholipid degradation and arachidonic acid mobilization in peritoneal macrophages. Biochim Biophys Acta. 1985; 846(2):265-74. DOI: 10.1016/0167-4889(85)90074-6. View

15.

Erman A, Baer P, Nasjletti A . Augmentation of unesterified arachidonic acid in the renal inner medulla of dexamethasone-treated rats. Relationship to altered triglyceride metabolism. J Biol Chem. 1985; 260(8):4679-83. View

16.

Jezyk P, Lands W . Specificity of acyl-CoA:phospholipid acyltransferases: solvent and temperature effects. J Lipid Res. 1968; 9(4):525-31. View

17.

Yamashita S, Nakaya N, Miki Y, Numa S . Separation of 1-acylglycerolphosphate acyltransferase and 1-acylglycerolphosphorylcholine acyltransferase of rat liver microsomes. Proc Natl Acad Sci U S A. 1975; 72(2):600-3. PMC: 432361. DOI: 10.1073/pnas.72.2.600. View

18.

Fels A, Pawlowski N, Cramer E, King T, COHN Z, Scott W . Human alveolar macrophages produce leukotriene B4. Proc Natl Acad Sci U S A. 1982; 79(24):7866-70. PMC: 347450. DOI: 10.1073/pnas.79.24.7866. View

19.

van den Bosch H . Intracellular phospholipases A. Biochim Biophys Acta. 1980; 604(2):191-246. DOI: 10.1016/0005-2736(80)90574-x. View

20.

Yamashita S, Hosaka K, Numa S . Acyl-donor specificities of partially purified 1-acylglycerophosphate acyltransferase, 2-acylglycerophosphate acyltransferase and 1-acylglycerophosphorylcholine acyltransferase from rat-liver microsomes. Eur J Biochem. 1973; 38(1):25-31. DOI: 10.1111/j.1432-1033.1973.tb03028.x. View