Genetic Modification of to Construct Stearidonic Acid Producing Cell Factory

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2019 Apr 17

PMID 30987311

Citations 14

Authors

Md Ahsanul Kabir Khan

Junhuan Yang

Syed Ammar Hussain

Huaiyuan Zhang

Victoriano Garre

Yuanda Song

Affiliations

Soon will be listed here.

Abstract

Stearidonic acid (SDA; 18:4, n-3) is the delta 15-desaturase product of gamma linolenic acid (GLA; 18:3, n-6) and delta 6-desaturase product of alpha linolenic acid (ALA; 18:3, n-3). Construction of engineered oleaginous microbes have been attracting significant interest in producing SDA because of its nutritional value and pharmaceutical applications. is a GLA producing filamentous fungus, which can be a useful tool to produce SDA. This study has, therefore, overexpressed the () gene from in this fungus to construct a SDA-producing cell factory. To produce SDA in , the homologous overexpression of gene was analyzed. When the gene was overexpressed in CBS 277.49, up to 5.0% SDA was accumulated in this strain. According to current knowledge, this is the first study describing the construction of a SDA-producing cell factory by overexpression of gene in oleaginous fungus . A new scope for further research has been established by this work to improve SDA production in this fungus, specifically in its high lipid-producing strain, WJ11.

Citing Articles

Simultaneous overexpression of ∆6-, ∆12- and ∆9-desaturases enhanced the production of γ-linolenic acid in WJ11.

Wang X, Yang J, Mohamed H, Shah A, Li S, Pang S Front Microbiol. 2023; 13:1078157.

PMID: 36590442 PMC: 9797528. DOI: 10.3389/fmicb.2022.1078157.

Genetic Manipulation in Mucorales and New Developments to Study Mucormycosis.

Lax C, Canovas-Marquez J, Tahiri G, Navarro E, Garre V, Nicolas F Int J Mol Sci. 2022; 23(7).

PMID: 35408814 PMC: 8998210. DOI: 10.3390/ijms23073454.

Microbes: A Hidden Treasure of Polyunsaturated Fatty Acids.

Shah A, Yang W, Mohamed H, Zhang Y, Song Y Front Nutr. 2022; 9:827837.

PMID: 35369055 PMC: 8968027. DOI: 10.3389/fnut.2022.827837.

Role of Cytosolic Malic Enzyme in Oleaginicity of High-Lipid-Producing Fungal Strain WJ11.

Fazili A, Shah A, Naz T, Nosheen S, Yang W, Garre V J Fungi (Basel). 2022; 8(3).

PMID: 35330267 PMC: 8955760. DOI: 10.3390/jof8030265.

Mucor circinelloides: a model organism for oleaginous fungi and its potential applications in bioactive lipid production.

Fazili A, Shah A, Zan X, Naz T, Nosheen S, Nazir Y Microb Cell Fact. 2022; 21(1):29.

PMID: 35227264 PMC: 8883733. DOI: 10.1186/s12934-022-01758-9.

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