The RNA Binding Activity of the First Identified Trypanosome Protein with Z-DNA-binding Domains

Overview

Journal Sci Rep

Specialty Science

Date 2019 Apr 13

PMID 30976048

Citations 10

Authors

Najmeh Nikpour

Reza Salavati

Affiliations

Soon will be listed here.

Abstract

RNA-binding proteins play a particularly important role in regulating gene expression in trypanosomes. A map of the network of protein complexes in Trypanosoma brucei uncovered an essential protein (Tb927.10.7910) that is postulated to be an RNA-binding protein implicated in the regulation of the mitochondrial post-transcriptional gene regulatory network by its association with proteins that participate in a multi-protein RNA editing complex. However, the mechanism by which this protein interacts with its multiple target transcripts remained unknown. Using sensitive database searches and experimental data, we identify Z-DNA-binding domains in T. brucei in the N- and C-terminal regions of Tb927.10.7910. RNA-binding studies of the wild-type protein, now referred to as RBP7910 (RNA binding protein 7910), and site-directed mutagenesis of residues important for the Z-DNA binding domains show that it preferentially interacts with RNA molecules containing poly(U) and poly(AU)-rich sequences. The interaction of RBP7910 with these regions may be involved in regulation of RNA editing of mitochondrial transcripts.

Citing Articles

Structure-Function Analysis of RBP7910: An Editosome Z-Binding Protein in Trypanosomatids.

Ehlert C, Poorinmohammad N, Mohammaei S, Zhang L, Salavati R Molecules. 2023; 28(19).

PMID: 37836806 PMC: 10574248. DOI: 10.3390/molecules28196963.

Zα domain proteins mediate the immune response.

Zhong Y, Zhong X, Qiao L, Wu H, Liu C, Zhang T Front Immunol. 2023; 14:1241694.

PMID: 37771585 PMC: 10523160. DOI: 10.3389/fimmu.2023.1241694.

Novel insights into double-stranded RNA-mediated immunopathology.

de Reuver R, Maelfait J Nat Rev Immunol. 2023; 24(4):235-249.

PMID: 37752355 DOI: 10.1038/s41577-023-00940-3.

Z-DNA and Z-RNA: Methods-Past and Future.

Herbert A Methods Mol Biol. 2023; 2651:295-329.

PMID: 36892776 DOI: 10.1007/978-1-0716-3084-6_21.

ADAR1 and ZBP1 in innate immunity, cell death, and disease.

Karki R, Kanneganti T Trends Immunol. 2023; 44(3):201-216.

PMID: 36710220 PMC: 9974732. DOI: 10.1016/j.it.2023.01.001.

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