A Novel Heterogalactan from Antrodia Camphorata and Anti-Angiogenic Activity of Its Sulfated Derivative

Overview

Journal Polymers (Basel)

Publisher MDPI

Date 2019 Apr 12

PMID 30970906

Citations 3

Authors

Yanqiu Liu

Yaqi Ding

Min Ye

Tao Zhu

Danbi Tian

Kan Ding

Affiliations

Soon will be listed here.

Abstract

A heterogalactan, named ACW0, was extracted from and purified by anion exchange and gel permeation chromatography. It was composed of galactose (94.98%), traces of mannose (2.41%), and fucose (2.61%), with its molecular weight estimated to be 13.5 k Da. The polysaccharide ACW0 was shown to be a mannofucogalactan with a backbone chain of α-d-1,6-linked Gal, attached by a non-reducing terminal α-d-Man and α-l-Fuc on C-2 of nearly every six α-d-1,6-linked Gal residues. A sulfated polysaccharide, ACW0-Sul was achieved by the chlorosulfonic acid-pyridine method. Compared with the native polysaccharide, ACW0-Sul could disrupt tube formation and migration as well as cell growth of human microvascular endothelial cells (HMEC-1) dose-dependently. Further studies revealed that phosphorylation of Extracellular Regulated Protein Kinases (Erk) and Focal Adhesion Kinase (FAK) were significantly inhibited by ACW0-Sul. These results suggested that ACW0-Sul could be a potent candidate for anti-angiogenic agent development.

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References

Alquini G, Carbonero E, Rosado F, Cosentino C, Iacomini M . Polysaccharides from the fruit bodies of the basidiomycete Laetiporus sulphureus (Bull.: Fr.) Murr. FEMS Microbiol Lett. 2004; 230(1):47-52. DOI: 10.1016/S0378-1097(03)00853-X. View

Haines A . Synthesis of 6,6'-ether linked disaccharides from D-allose, D-galactose, D-glucose and D-mannose; evidence on the structure of coyolosa. Org Biomol Chem. 2004; 2(16):2352-8. DOI: 10.1039/b407468h. View

Cheng J, Huang N, Chang T, Wang D, Lu M . Study for anti-angiogenic activities of polysaccharides isolated from Antrodia cinnamomea in endothelial cells. Life Sci. 2005; 76(26):3029-42. DOI: 10.1016/j.lfs.2004.11.023. View

Hseu Y, Wu F, Wu J, Chen J, Chang W, Lu F . Anti-inflammatory potential of Antrodia Camphorata through inhibition of iNOS, COX-2 and cytokines via the NF-kappaB pathway. Int Immunopharmacol. 2005; 5(13-14):1914-25. DOI: 10.1016/j.intimp.2005.06.013. View

Yang H, Chen C, Chang W, Lu F, Lai Y, Chen C . Growth inhibition and induction of apoptosis in MCF-7 breast cancer cells by Antrodia camphorata. Cancer Lett. 2006; 231(2):215-27. DOI: 10.1016/j.canlet.2005.02.004. View

Fan J, Zhang J, Tang Q, Liu Y, Zhang A, Pan Y . Structural elucidation of a neutral fucogalactan from the mycelium of Coprinus comatus. Carbohydr Res. 2006; 341(9):1130-4. DOI: 10.1016/j.carres.2006.03.039. View

Huang X, Wang D, Hu Y, Lu Y, Guo Z, Kong X . Effect of sulfated astragalus polysaccharide on cellular infectivity of infectious bursal disease virus. Int J Biol Macromol. 2007; 42(2):166-71. DOI: 10.1016/j.ijbiomac.2007.10.019. View

Wang L, Huang H, Wei Y, Li X, Chen Z . Characterization and anti-tumor activities of sulfated polysaccharide SRBPS2a obtained from defatted rice bran. Int J Biol Macromol. 2009; 45(4):427-31. DOI: 10.1016/j.ijbiomac.2009.06.006. View

Wang J, Hu Y, Wang D, Liu J, Zhang J, Abula S . Sulfated modification can enhance the immune-enhancing activity of lycium barbarum polysaccharides. Cell Immunol. 2010; 263(2):219-23. DOI: 10.1016/j.cellimm.2010.04.001. View

10.

Qiu H, Yang B, Pei Z, Zhang Z, Ding K . WSS25 inhibits growth of xenografted hepatocellular cancer cells in nude mice by disrupting angiogenesis via blocking bone morphogenetic protein (BMP)/Smad/Id1 signaling. J Biol Chem. 2010; 285(42):32638-46. PMC: 2952266. DOI: 10.1074/jbc.M110.105544. View

11.

Cho S, Yun B, Yoo I, Koshino H . Structure of fomitellan A, a mannofucogalactan from the fruiting bodies of Fomitella fraxinea. Bioorg Med Chem Lett. 2010; 21(1):204-6. DOI: 10.1016/j.bmcl.2010.11.036. View

12.

Lechertier T, Hodivala-Dilke K . Focal adhesion kinase and tumour angiogenesis. J Pathol. 2011; 226(2):404-12. DOI: 10.1002/path.3018. View

13.

Wei D, Wei Y, Cheng W, Zhang L . Sulfated modification, characterization and antitumor activities of Radix hedysari polysaccharide. Int J Biol Macromol. 2012; 51(4):471-6. DOI: 10.1016/j.ijbiomac.2012.06.004. View

14.

Morimoto M, Takatori M, Hayashi T, Mori D, Takashima O, Yoshida S . Depolymerization of sulfated polysaccharides under hydrothermal conditions. Carbohydr Res. 2013; 384:56-60. DOI: 10.1016/j.carres.2013.11.017. View

15.

Shi M, Yang Y, Hu X, Zhang Z . Effect of ultrasonic extraction conditions on antioxidative and immunomodulatory activities of a Ganoderma lucidum polysaccharide originated from fermented soybean curd residue. Food Chem. 2014; 155:50-6. DOI: 10.1016/j.foodchem.2014.01.037. View

16.

Li X, Xiao J, Zha X, Pan L, Asghar M, Luo J . Structural identification and sulfated modification of an antiglycation Dendrobium huoshanense polysaccharide. Carbohydr Polym. 2014; 106:247-54. DOI: 10.1016/j.carbpol.2014.02.029. View

17.

Cong Q, Shang M, Dong Q, Liao W, Xiao F, Ding K . Structure and activities of a novel heteroxylan from Cassia obtusifolia seeds and its sulfated derivative. Carbohydr Res. 2014; 393:43-50. DOI: 10.1016/j.carres.2014.04.016. View

18.

Wang P, Zhang L, Yao J, Shi Y, Li P, Ding K . An arabinogalactan from flowers of Panax notoginseng inhibits angiogenesis by BMP2/Smad/Id1 signaling. Carbohydr Polym. 2015; 121:328-35. DOI: 10.1016/j.carbpol.2014.11.073. View

19.

Wang W, Chen H, Zhang L, Qin Y, Cong Q, Wang P . A fucoidan from Nemacystus decipiens disrupts angiogenesis through targeting bone morphogenetic protein 4. Carbohydr Polym. 2016; 144:305-14. DOI: 10.1016/j.carbpol.2016.02.068. View

20.

Zheng X, Bao W, Yang J, Zhang T, Sun D, Liang Y . Focal Adhesion Kinase Directly Interacts with TSC2 Through Its FAT Domain and Regulates Cell Proliferation in Cashmere Goat Fetal Fibroblasts. DNA Cell Biol. 2016; 35(9):480-8. DOI: 10.1089/dna.2015.3033. View