Identification of a Novel Anti-cancer Protein, FIP-bbo, from Botryobasidium Botryosum and Protein Structure Analysis Using Molecular Dynamic Simulation

Overview

Journal Sci Rep

Specialty Science

Date 2019 Apr 11

PMID 30967569

Citations 7

Authors

Ying Wang

Ying Nv Gao

Rui Bai

Hong Yu Chen

Ying Ying Wu

Jun Jun Shang

Da Peng Bao

Affiliations

Soon will be listed here.

Abstract

Fungal immunoregulatory proteins (FIP) are effective small molecule proteins with broad-spectrum immunomodulatory and anti-cancer activities and can be potential agents for the development of clinical drugs and health food additives. In this study, a new member of FIP named FIP-bbo was obtained through Botryobasidium botryosum genome mining. FIP-bbo has the typical characteristics of FIP but is genetically distant from other FIPs. Recombinant FIP-bbo (rFIP-bbo) was produced in an optimized E. coli expression system, and the pure protein was isolated using a Ni-NTA column. Antineoplastic experiments suggested that FIP-bbo is similar to LZ-8 in inhibiting various cancer cells (Hela, Spac-1, and A549) at lower concentrations, but it is not as potent as LZ-8. The molecular mechanism by which FIP-bbo, FIP-fve, and LZ-8 are cytotoxic to cancer cells has been discussed based on molecular dynamics simulation. Point mutations that may improve the thermal stability of FIP-fve and FIP-bbo were predicted. These results not only present a new candidate protein for the development of anticancer adjuvants, but also provide an approach for designing FIPs with high anticancer activity.

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