Korean Red Ginseng Enhances Pneumococcal Δ Vaccine Efficacy by Inhibiting Reactive Oxygen Species Production

Overview

Journal J Ginseng Res

Date 2019 Apr 10

PMID 30962736

Citations 6

Authors

Si-On Lee

Seungyeop Lee

Se-Jin Kim

Dong-Kwon Rhee

Affiliations

Soon will be listed here.

Abstract

Background: , more than 90 serotypes of which exist, is recognized as an etiologic agent of pneumonia, meningitis, and sepsis associated with significant morbidity and mortality worldwide. Immunization with a pneumococcal mutant (Δ) has been shown to confer comprehensive, long-term protection against even nontypeable strains. However, Δ is effective as a vaccine only after at least three rounds of immunization. Therefore, treatments capable of enhancing the efficiency of Δ immunization should be identified without delay. Mayer has already been shown to have pharmacological and antioxidant effects. Here, the ability of Korean Red Ginseng (KRG) to enhance the efficacy of Δ immunization was investigated.

Methods: Mice were treated with KRG and immunized with Δ before infection with the pathogenic strain D39. Total reactive oxygen species production was measured using lung homogenates, and inducible nitric oxide (NO) synthase and antiapoptotic protein expression was determined by immunoblotting. The phagocytic activity of peritoneal macrophages was also tested after KRG treatment.

Results: Compared with the other treatments, KRG significantly increased survival rate after lethal challenge and resulted in faster bacterial clearance via increased phagocytosis. Moreover, KRG enhanced Δ vaccine efficacy by inhibiting reactive oxygen species production, reducing extracellular signal-regulated kinase apoptosis signaling and inflammation.

Conclusion: Taken together, our results suggest that KRG reduces the time required for immunization with the Δ vaccine by enhancing its efficacy.

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References

Saura M, Zaragoza C, Bao C, McMillan A, Lowenstein C . Interaction of interferon regulatory factor-1 and nuclear factor kappaB during activation of inducible nitric oxide synthase transcription. J Mol Biol. 1999; 289(3):459-71. DOI: 10.1006/jmbi.1999.2752. View

Hvalbye B, Aaberge I, Lovik M, Haneberg B . Intranasal immunization with heat-inactivated Streptococcus pneumoniae protects mice against systemic pneumococcal infection. Infect Immun. 1999; 67(9):4320-5. PMC: 96747. DOI: 10.1128/IAI.67.9.4320-4325.1999. View

Fedson D, Scott J, Scott G . The burden of pneumococcal disease among adults in developed and developing countries: what is and is not known. Vaccine. 1999; 17 Suppl 1:S11-8. DOI: 10.1016/s0264-410x(99)00122-x. View

Wang X, Martindale J, Holbrook N . Requirement for ERK activation in cisplatin-induced apoptosis. J Biol Chem. 2000; 275(50):39435-43. DOI: 10.1074/jbc.M004583200. View

Briles D, Hollingshead S, Nabors G, Paton J . The potential for using protein vaccines to protect against otitis media caused by Streptococcus pneumoniae. Vaccine. 2001; 19 Suppl 1:S87-95. DOI: 10.1016/s0264-410x(00)00285-1. View

Bacus S, Gudkov A, Lowe M, Lyass L, Yung Y, Komarov A . Taxol-induced apoptosis depends on MAP kinase pathways (ERK and p38) and is independent of p53. Oncogene. 2001; 20(2):147-55. DOI: 10.1038/sj.onc.1204062. View

Miyaji E, Dias W, Gamberini M, Gebara V, Schenkman R, Wild J . PsaA (pneumococcal surface adhesin A) and PspA (pneumococcal surface protein A) DNA vaccines induce humoral and cellular immune responses against Streptococcus pneumoniae. Vaccine. 2001; 20(5-6):805-12. DOI: 10.1016/s0264-410x(01)00395-4. View

Teng X, Zhang H, Snead C, Catravas J . Molecular mechanisms of iNOS induction by IL-1 beta and IFN-gamma in rat aortic smooth muscle cells. Am J Physiol Cell Physiol. 2001; 282(1):C144-52. DOI: 10.1152/ajpcell.2002.282.1.C144. View

Tang D, Wu D, Hirao A, Lahti J, Liu L, Mazza B . ERK activation mediates cell cycle arrest and apoptosis after DNA damage independently of p53. J Biol Chem. 2002; 277(15):12710-7. DOI: 10.1074/jbc.M111598200. View

10.

Ramachandiran S, Huang Q, Dong J, Lau S, Monks T . Mitogen-activated protein kinases contribute to reactive oxygen species-induced cell death in renal proximal tubule epithelial cells. Chem Res Toxicol. 2002; 15(12):1635-42. DOI: 10.1021/tx0200663. View

11.

Mutsch M, Zhou W, Rhodes P, Bopp M, Chen R, Linder T . Use of the inactivated intranasal influenza vaccine and the risk of Bell's palsy in Switzerland. N Engl J Med. 2004; 350(9):896-903. DOI: 10.1056/NEJMoa030595. View

12.

Bogaert D, de Groot R, Hermans P . Streptococcus pneumoniae colonisation: the key to pneumococcal disease. Lancet Infect Dis. 2004; 4(3):144-54. DOI: 10.1016/S1473-3099(04)00938-7. View

13.

Bogaert D, Hermans P, Adrian P, Rumke H, de Groot R . Pneumococcal vaccines: an update on current strategies. Vaccine. 2004; 22(17-18):2209-20. DOI: 10.1016/j.vaccine.2003.11.038. View

14.

Chen M, Bao W, Aizman R, Huang P, Aspevall O, Gustafsson L . Activation of extracellular signal-regulated kinase mediates apoptosis induced by uropathogenic Escherichia coli toxins via nitric oxide synthase: protective role of heme oxygenase-1. J Infect Dis. 2004; 190(1):127-35. DOI: 10.1086/421243. View

15.

Dong J, Ramachandiran S, Tikoo K, Jia Z, Lau S, Monks T . EGFR-independent activation of p38 MAPK and EGFR-dependent activation of ERK1/2 are required for ROS-induced renal cell death. Am J Physiol Renal Physiol. 2004; 287(5):F1049-58. DOI: 10.1152/ajprenal.00132.2004. View

16.

Cutts F, Zaman S, Enwere G, Jaffar S, Levine O, Okoko J . Efficacy of nine-valent pneumococcal conjugate vaccine against pneumonia and invasive pneumococcal disease in The Gambia: randomised, double-blind, placebo-controlled trial. Lancet. 2005; 365(9465):1139-46. DOI: 10.1016/S0140-6736(05)71876-6. View

17.

Giudice E, Campbell J . Needle-free vaccine delivery. Adv Drug Deliv Rev. 2006; 58(1):68-89. DOI: 10.1016/j.addr.2005.12.003. View

18.

Zhuang S, Yan Y, Daubert R, Han J, Schnellmann R . ERK promotes hydrogen peroxide-induced apoptosis through caspase-3 activation and inhibition of Akt in renal epithelial cells. Am J Physiol Renal Physiol. 2006; 292(1):F440-7. DOI: 10.1152/ajprenal.00170.2006. View

19.

Oosterhuis-Kafeja F, Beutels P, Van Damme P . Immunogenicity, efficacy, safety and effectiveness of pneumococcal conjugate vaccines (1998-2006). Vaccine. 2007; 25(12):2194-212. DOI: 10.1016/j.vaccine.2006.11.032. View

20.

Roche A, King S, Weiser J . Live attenuated Streptococcus pneumoniae strains induce serotype-independent mucosal and systemic protection in mice. Infect Immun. 2007; 75(5):2469-75. PMC: 1865756. DOI: 10.1128/IAI.01972-06. View