The Rate of CD4 T Cell Entry into the Lungs During Mycobacterium Tuberculosis Infection Is Determined by Partial and Opposing Effects of Multiple Chemokine Receptors

The specific chemokine receptors utilized by Th1 cells to migrate into the lung during infection are unknown. We previously showed in mice that CXCR3 Th1 cells enter the lung parenchyma and suppress growth, while CX3CR1 KLRG1 Th1 cells accumulate in the lung vasculature and are nonprotective. Here we quantify the contributions of these chemokine receptors to the migration and entry rate of Th1 cells into -infected lungs using competitive adoptive transfer migration assays and mathematical modeling. We found that in 8.6 h half of -specific CD4 T cells migrate from the blood to the lung parenchyma. CXCR3 deficiency decreases the average rate of Th1 cell entry into the lung parenchyma by half, while CX3CR1 deficiency doubles it. KLRG1 blockade has no effect on Th1 cell lung migration. CCR2, CXCR5, and, to a lesser degree, CCR5 and CXCR6 also promote the entry of Th1 cells into the lungs of infected mice. Moreover, blockade of G-protein-coupled receptors with pertussis toxin treatment prior to transfer only partially inhibits T cell migration into the lungs. Thus, the fraction of Th1 cell input into the lungs during infection that is regulated by chemokine receptors likely reflects the cumulative effects of multiple chemokine receptors that mostly promote but that can also inhibit entry into the parenchyma.