Estrogen Mitigates the Negative Effects of Arsenic Contamination in an Wound Model

Overview

Journal Appl In Vitro Toxicol

Date 2019 Apr 9

PMID 30956995

Citations 4

Authors

Bronson I Pinto

Oscar R Lujan

Stephan A Ramos

Catherine R Propper

Robert S Kellar

Affiliations

Soon will be listed here.

Abstract

Arsenic, a naturally occurring environmental contaminant, is harmful to humans at elevated concentrations. Increased levels of arsenic in the environment occur as a result of human activities and from natural geologically sourced leaching into ground and surface water. These sources pose an exposure risk above the USEPA standard to individuals whose food and water sources become contaminated. Arsenic exposure negatively impacts organ function and increases the risk for developing pathologies, including cancer. Some of the effects of arsenic on cancer translate to normal cell function in wound healing. To evaluate whether arsenic influences wound healing, an scratch assay was employed to study the effects of arsenic on cellular migration, which is a key component in the normal wound-healing process. In this study, skin cells were exposed to environmentally relevant concentrations of arsenic, and wound closure was evaluated. Results indicated that arsenic significantly decreased the rate of cellular migration in the scratch assay when compared with controls. In addition, estradiol, which has been shown to positively influence cellular and tissue processes involved in wound healing, reversed the slowing effects of arsenic on wound closure. These results suggest that arsenic contamination may inhibit, and estrogen may provide a therapeutic benefit for individuals with arsenic-contaminated wounds.

Citing Articles

Arsenic Impairs Wound Healing Processes in Dermal Fibroblasts and Mice.

Dresler S, Pinto B, Salanga M, Propper C, Berry S, Kellar R Int J Mol Sci. 2024; 25(4).

PMID: 38396835 PMC: 10888720. DOI: 10.3390/ijms25042161.

Therapeutic candidates for keloid scars identified by qualitative review of scratch assay research for wound healing.

Alishahedani M, Yadav M, McCann K, Gough P, Castillo C, Matriz J PLoS One. 2021; 16(6):e0253669.

PMID: 34143844 PMC: 8213172. DOI: 10.1371/journal.pone.0253669.

Cytotoxicity, Cell Cycle Arrest, and Apoptosis Induction Activity of Ethyl-p-methoxycinnamate in Cholangiocarcinoma Cell.

Muhamad P, Panrit L, Chaijaroenkul W, Na-Bangchang K Asian Pac J Cancer Prev. 2020; 21(4):927-934.

PMID: 32334452 PMC: 7445962. DOI: 10.31557/APJCP.2020.21.4.927.

In Vitro Scratch Assay to Demonstrate Effects of Arsenic on Skin Cell Migration.

Pinto B, Cruz N, Lujan O, Propper C, Kellar R J Vis Exp. 2019; (144).

PMID: 30855562 PMC: 7537821. DOI: 10.3791/58838.

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