Up-regulation of Grb2-associated Binder 1 Promotes Hepatocyte Growth Factor-induced Endothelial Progenitor Cell Proliferation and Migration

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2019 Apr 9

PMID 30956905

Authors

Qing Fan

Liyu Zhang

Wenjie Zhu

Sheng Xue

Yisheng Song

Qing Chang

Affiliations

Soon will be listed here.

Abstract

Objectives: Grb2-associated binder 1 (Gab1), a scaffolding adaptor protein, plays an important role in transmitting key signals that control cell growth, migration, and function from multiple tyrosine kinase receptors. This study was designed to investigate the influence of upregulation of Gab1 in endothelial progenitor cells (EPCs) stimulated with hepatocyte growth factor (HGF), and the underlying molecular mechanisms.

Materials And Methods: Endothelial progenitor cells isolated from human umbilical cord blood were identified and divided into four groups. EPCs in the Control group were cultured normally; those in the Control+HGF group were treated with HGF stimulation; those in the AD-Gab1 group were transfected with adenovirus containing the gene but not treated with HGF stimulation; and, those in the AD-Gab1+HGF group were treated with both HGF stimulation and transfection with adenovirus containing the gene. Subsequently, expression and proliferation and migration ability were compared for EPCs grown under different conditions. Furthermore, we measured phosphorylation levels of three key proteins Gab1, SHP2, and ERK1/2.

Results: The AD-Gab1+HGF group had the highest expression of Gab1 and higher proliferation and migration than the other three groups.

Conclusions: Upregulation of Gab1 promoted HGF-induced EPC proliferation and migration. Mechanistically, HGF stimulated Gab1 tyrosine phosphorylation in EPCs, thus leading to activation of extracellular regulated MAP kinase 1/2, which is involved in proliferation and migration signaling.

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