Construction of DGLA Producing Cell Factory by Genetic Modification of Mucor Circinelloides

Overview

Journal Microb Cell Fact

Publisher Biomed Central

Specialties Biotechnology
Microbiology

Date 2019 Apr 5

PMID 30943965

Citations 16

Authors

Md Ahsanul Kabir Khan

Junhuan Yang

Syed Ammar Hussain

Huaiyuan Zhang

Li Liang

Victoriano Garre

Yuanda Song

Affiliations

Soon will be listed here.

Abstract

Background: Dihomo-gamma linolenic acid (DGLA, 20:3, n-6) is the elongated product of Gamma linolenic acid (GLA, 18:3, n-6) catalyzed by the enzyme delta-6 elongase (D6E) or gamma linolenic acid elongase (GLELO). Construction of engineered oleaginous microbes have been attracting significant interest to produce DGLA because of its nutritional value and medicinal applications. Mucor circinelloides is a GLA producing filamentous fungus which can be a useful tool to produce DGLA. We have, therefore, overexpressed the D6E (GLELO) gene in this fungus to construct DGLA producing cell factory.

Result: To produce DGLA in M. circinelloides, homologous overexpression of D6E (GLELO) gene was analyzed. When the gene was overexpressed in M. circinelloides CBS277.49, up to 5.72% DGLA was produced in this strain.

Conclusion: To our knowledge, this is the first report describing the overexpression of D6E (GLELO) gene in M. circinelloides to construct DGLA producing cell factory. A new scope for further research has been established by this work for improved production of DGLA in this fungus, specifically in its high lipid-producing strain, WJ11.

Citing Articles

Simultaneous overexpression of ∆6-, ∆12- and ∆9-desaturases enhanced the production of γ-linolenic acid in WJ11.

Wang X, Yang J, Mohamed H, Shah A, Li S, Pang S Front Microbiol. 2023; 13:1078157.

PMID: 36590442 PMC: 9797528. DOI: 10.3389/fmicb.2022.1078157.

Evaluation of Different Standard Amino Acids to Enhance the Biomass, Lipid, Fatty Acid, and γ-Linolenic Acid Production in and .

Mohamed H, Awad M, Shah A, Nazir Y, Naz T, Hassane A Front Nutr. 2022; 9:876817.

PMID: 35592629 PMC: 9112836. DOI: 10.3389/fnut.2022.876817.

Genetic Manipulation in Mucorales and New Developments to Study Mucormycosis.

Lax C, Canovas-Marquez J, Tahiri G, Navarro E, Garre V, Nicolas F Int J Mol Sci. 2022; 23(7).

PMID: 35408814 PMC: 8998210. DOI: 10.3390/ijms23073454.

Microbes: A Hidden Treasure of Polyunsaturated Fatty Acids.

Shah A, Yang W, Mohamed H, Zhang Y, Song Y Front Nutr. 2022; 9:827837.

PMID: 35369055 PMC: 8968027. DOI: 10.3389/fnut.2022.827837.

Mucor circinelloides: a model organism for oleaginous fungi and its potential applications in bioactive lipid production.

Fazili A, Shah A, Zan X, Naz T, Nosheen S, Nazir Y Microb Cell Fact. 2022; 21(1):29.

PMID: 35227264 PMC: 8883733. DOI: 10.1186/s12934-022-01758-9.

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