Activation of Human B Lymphocytes Induced by Robinia Pseudoacacia Lectin in the Presence of T Cells

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1978 Oct 1

PMID 309432

Authors

A Sharif

Lethibichthuy

J BROCHIER

Y Goussault

R Bourrillon

Affiliations

Soon will be listed here.

Abstract

Robinia pseudoacacia seed lectin is a potent human lymphocyte activator which is capable of activating pure T cells but not pure B lymphocytes. However, when B and T cells were cultured together, the thymidine incorporation was found to be higher than that expected from B- or T-cell cultures alone. Killing of T cells by anti-human-T-lymphocyte antigen (HTLA) serum and complement at the time of thymidine incorporation was found to be unable to suppress completely the thymidine uptake whereas treatment by anti-human-B-lymphocyte and monocyte antigen (HBLMA) serum reduced the response to some extent. Moreover, stimulated lymphoblasts were shown to bear B-cell markers (surface Ig and complement receptors) in about the same proportion as B lymphocytes present in the cultures. These results show that B cells proliferate in the presence of T cells and Robinia lectin. Finally, activation of B cells by Robinia lectin in the presence of T cells led to their maturation to plasma cells in the same way as PWM.

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