KEOPS Complex Promotes Homologous Recombination Via DNA Resection

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2019 Apr 3

PMID 30937455

Citations 15

Authors

Ming-Hong He

Jia-Cheng Liu

Yi-Si Lu

Zhi-Jing Wu

Ying-Ying Liu

Zhenfang Wu

Jing Peng

Jin-Qiu Zhou

Affiliations

Soon will be listed here.

Abstract

KEOPS complex is one of the most conserved protein complexes in eukaryotes. It plays important roles in both telomere uncapping and tRNA N6-threonylcarbamoyladenosine (t6A) modification in budding yeast. But whether KEOPS complex plays any roles in DNA repair remains unknown. Here, we show that KEOPS complex plays positive roles in both DNA damage response and homologous recombination-mediated DNA repair independently of its t6A synthesis function. Additionally, KEOPS displays DNA binding activity in vitro, and is recruited to the chromatin at DNA breaks in vivo, suggesting a direct role of KEOPS in DSB repair. Mechanistically, KEOPS complex appears to promote DNA end resection through facilitating the association of Exo1 and Dna2 with DNA breaks. Interestingly, inactivation of both KEOPS and Mre11/Rad50/Xrs2 (MRX) complexes results in synergistic defect in DNA resection, revealing that KEOPS and MRX have some redundant functions in DNA resection. Thus we uncover a t6A-independent role of KEOPS complex in DNA resection, and propose that KEOPS might be a DSB sensor to assist cells in maintaining chromosome stability.

Citing Articles

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The archaeal KEOPS complex possesses a functional Gon7 homolog and has an essential function independent of the cellular tA modification level.

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Elimination of subtelomeric repeat sequences exerts little effect on telomere essential functions in .

Hu C, Zhu X, He M, Shao Y, Qin Z, Wu Z Elife. 2024; 12.

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