Development of a Colloidal Gold-based Immunochromatographic Strip for Rapid Detection of Rice Stripe Virus

Overview

Journal J Zhejiang Univ Sci B

Specialties Biology
General Medicine

Date 2019 Apr 2

PMID 30932379

Citations 10

Authors

De-Qing Huang

Rui Chen

Ya-Qin Wang

Jian Hong

Xue-Ping Zhou

Jian-Xiang Wu

Affiliations

Soon will be listed here.

Abstract

Rice stripe virus (RSV) causes dramatic losses in rice production worldwide. In this study, two monoclonal antibodies (MAbs) 16E6 and 11C1 against RSV and a colloidal gold-based immunochromatographic strip were developed for specific, sensitive, and rapid detection of RSV in rice plant and planthopper samples. The MAb 16E6 was conjugated with colloidal gold and the MAb 11C1 was coated on the test line of the nitrocellulose membrane of the test strip. The specificity of the test strip was confirmed by a positive reaction to RSV-infected rice plants and small brown planthopper (SBPH), and negative reactions to five other rice viruses, healthy rice plants, four other vectors of five rice viruses, and non-viruliferous SBPH. Sensitivity analyses showed that the test strip could detect the virus in RSV-infected rice plant tissue crude extracts diluted to 1:20 480 (w/v, g/mL), and in individual viruliferous SBPH homogenate diluted to 1:2560 (individual SPBH/μL). The validity of the developed strip was further confirmed by tests using field-collected rice and SBPH samples. This newly developed test strip is a low-cost, fast, and easy-to-use tool for on-site detection of RSV infection during field epidemiological studies and paddy field surveys, and thus can benefit decision-making for RSV management in the field.

Citing Articles

Development of a colloidal gold-based immunochromatographic strip for rapid detection of .

Lu J, He W, Liu Y, Li S, Huang X, Wang H Front Microbiol. 2025; 16:1533170.

PMID: 40066268 PMC: 11892987. DOI: 10.3389/fmicb.2025.1533170.

Unleashing the power of colloidal gold immunochromatographic assays for plant virus diagnostics.

Ghorbani A, Astaraki S, Rostami M, Pakdel A MethodsX. 2023; 12:102498.

PMID: 38089155 PMC: 10714363. DOI: 10.1016/j.mex.2023.102498.

Visual and Super-Sensitive Detection of Maize Chlorotic Mottle Virus by Dot-ELISA and Au Nanoparticle-Based Immunochromatographic Test Strip.

Zhang C, Guo M, Dong J, Liu L, Zhou X, Wu J Viruses. 2023; 15(7).

PMID: 37515293 PMC: 10383747. DOI: 10.3390/v15071607.

Molecular Hydrogen Confers Resistance to Rice Stripe Virus.

Shao Y, Lin F, Wang Y, Cheng P, Lou W, Wang Z Microbiol Spectr. 2023; :e0441722.

PMID: 36840556 PMC: 10100981. DOI: 10.1128/spectrum.04417-22.

Development of Dot-ELISA and Colloidal Gold Immunochromatographic Strip for Rapid and Super-Sensitive Detection of Plum Pox Virus in Apricot Trees.

Guo M, Qi D, Dong J, Dong S, Yang X, Qian Y Viruses. 2023; 15(1).

PMID: 36680209 PMC: 9861805. DOI: 10.3390/v15010169.

References

Falk B, Tsai J . Biology and molecular biology of viruses in the genus Tenuivirus. Annu Rev Phytopathol. 2004; 36:139-63. DOI: 10.1146/annurev.phyto.36.1.139. View

Hibino H . Biology and epidemiology of rice viruses. Annu Rev Phytopathol. 1996; 34:249-74. DOI: 10.1146/annurev.phyto.34.1.249. View

Barbier P, Takahashi M, Nakamura I, Toriyama S, Ishihama A . Solubilization and promoter analysis of RNA polymerase from rice stripe virus. J Virol. 1992; 66(10):6171-4. PMC: 283666. DOI: 10.1128/JVI.66.10.6171-6174.1992. View

Zhu Y, Hayakawa T, Toriyama S . Complete nucleotide sequence of RNA 4 of rice stripe virus isolate T, and comparison with another isolate and with maize stripe virus. J Gen Virol. 1992; 73 ( Pt 5):1309-12. DOI: 10.1099/0022-1317-73-5-1309. View

Wang Y, Xue Y, Li J . Towards molecular breeding and improvement of rice in China. Trends Plant Sci. 2005; 10(12):610-4. DOI: 10.1016/j.tplants.2005.10.008. View

Zhang G, Wang X, Yang J, Yang Y, Xing G, Li Q . Development of an immunochromatographic lateral flow test strip for detection of beta-adrenergic agonist Clenbuterol residues. J Immunol Methods. 2006; 312(1-2):27-33. DOI: 10.1016/j.jim.2006.02.017. View

Zhang X, Wang X, Zhou G . A one-step real time RT-PCR assay for quantifying rice stripe virus in rice and in the small brown planthopper (Laodelphax striatellus Fallen). J Virol Methods. 2008; 151(2):181-187. DOI: 10.1016/j.jviromet.2008.05.024. View

Xiong R, Wu J, Zhou Y, Zhou X . Identification of a movement protein of the tenuivirus rice stripe virus. J Virol. 2008; 82(24):12304-11. PMC: 2593352. DOI: 10.1128/JVI.01696-08. View

Xiong R, Wu J, Zhou Y, Zhou X . Characterization and subcellular localization of an RNA silencing suppressor encoded by Rice stripe tenuivirus. Virology. 2009; 387(1):29-40. DOI: 10.1016/j.virol.2009.01.045. View

10.

Kakutani T, Hayano Y, Hayashi T, Minobe Y . Ambisense segment 3 of rice stripe virus: the first instance of a virus containing two ambisense segments. J Gen Virol. 1991; 72 ( Pt 2):465-8. DOI: 10.1099/0022-1317-72-2-465. View

11.

Wu J, Zhang S, Zhou X . Monoclonal antibody-based ELISA and colloidal gold-based immunochromatographic assay for streptomycin residue detection in milk and swine urine. J Zhejiang Univ Sci B. 2010; 11(1):52-60. PMC: 2801090. DOI: 10.1631/jzus.B0900215. View

12.

Zhu Y, Hayakawa T, Toriyama S, Takahashi M . Complete nucleotide sequence of RNA 3 of rice stripe virus: an ambisense coding strategy. J Gen Virol. 1991; 72 ( Pt 4):763-7. DOI: 10.1099/0022-1317-72-4-763. View

13.

Le D, Netsu O, Uehara-Ichiki T, Shimizu T, Choi I, Omura T . Molecular detection of nine rice viruses by a reverse-transcription loop-mediated isothermal amplification assay. J Virol Methods. 2010; 170(1-2):90-3. DOI: 10.1016/j.jviromet.2010.09.004. View

14.

Hayano Y, Kakutani T, Hayashi T, Minobe Y . Coding strategy of rice stripe virus: major nonstructural protein is encoded in viral RNA segment 4 and coat protein in RNA complementary to segment 3. Virology. 1990; 177(1):372-4. DOI: 10.1016/0042-6822(90)90493-b. View

15.

Xu Y, Zhou X . Role of Rice Stripe Virus NSvc4 in Cell-to-Cell Movement and Symptom Development in Nicotiana benthamiana. Front Plant Sci. 2012; 3:269. PMC: 3516811. DOI: 10.3389/fpls.2012.00269. View

16.

Perosa F, Carbone R, Ferrone S, Dammacco F . Purification of human immunoglobulins by sequential precipitation with caprylic acid and ammonium sulphate. J Immunol Methods. 1990; 128(1):9-16. DOI: 10.1016/0022-1759(90)90458-8. View

17.

Kuang H, Xing C, Hao C, Liu L, Wang L, Xu C . Rapid and highly sensitive detection of lead ions in drinking water based on a strip immunosensor. Sensors (Basel). 2013; 13(4):4214-24. PMC: 3673080. DOI: 10.3390/s130404214. View

18.

Wu G, Lu Y, Zheng H, Lin L, Yan F, Chen J . Transcription of ORFs on RNA2 and RNA4 of Rice stripe virus terminate at an AUCCGGAU sequence that is conserved in the genus Tenuivirus. Virus Res. 2013; 175(1):71-7. DOI: 10.1016/j.virusres.2013.04.009. View

19.

Huang L, Rao L, Zhou X, Wu J . Genetic variability and evolution of rice stripe virus. J Zhejiang Univ Sci B. 2013; 14(10):875-85. PMC: 3796639. DOI: 10.1631/jzus.B1300014. View

20.

Kong L, Wu J, Lu L, Xu Y, Zhou X . Interaction between Rice stripe virus disease-specific protein and host PsbP enhances virus symptoms. Mol Plant. 2013; 7(4):691-708. DOI: 10.1093/mp/sst158. View