Mitochondrial DNA and Genomic DNA Ratio in Embryo Culture Medium is Not a Reliable Predictor for in Vitro Fertilization Outcome

Overview

Journal Sci Rep

Specialty Science

Date 2019 Mar 31

PMID 30926852

Citations 3

Authors

Xinyue Zhang

Yue Sun

Xin Dong

Jianming Zhou

Fubo Sun

Tingting Han

Ping Lei

Rurong Mao

Xuzhou Guo

Qi Wang

Penghao Li

Ting Qu

Jihua Huang

Lingxiao Li

Tianhua Huang

Ying Zhong

Jiang Gu

Affiliations

Soon will be listed here.

Abstract

To investigate the ratio of mitochondrial DNA to genomic DNA (mt/gDNA) in embryo culture medium as a possible predictor for embryonic development and pregnancy outcome, we collected a total of 93 embryo biopsy specimens from 52 women at the corresponding Day 3 (D3) and Day 5 (D5) embryo culture medium of in vitro fertilization. With the multiple annealing and looping-based amplification cycles method of next-generation sequencing for whole genome amplification, we examined the karyotype of the biopsy samples and the mt/gDNA ratio in the culture medium. Results showed that the ratio of mt/gDNA had an upward trend with decreasing trophectoderm levels with no significant difference. At the same time, from D3 to D5, the mt/gDNA ratio in the medium of embryos that failed to become blastocysts showed an upward trend, and the mt/gDNA ratio of medium from embryos that reached blastulation with successful pregnancy showed a decreasing trend, but the differences were not statistically significant. We conclude that there is a certain correlation between mt/gDNA ratio and early embryonic development, but it does not reach a level that can be used as a clinical predictor.

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