Alkaline and Halophilic Protease Production by H11 and Its Potential Industrial Applications

Overview

Journal Food Technol Biotechnol

Specialty Biotechnology

Date 2019 Mar 30

PMID 30923452

Citations 13

Authors

Agnieszka Kalwasinska

Urszula Jankiewicz

Tamas Felfoldi

Aleksandra Burkowska-But

Maria Swiontek Brzezinska

Affiliations

Soon will be listed here.

Abstract

This paper presents the results of the study on the production of protease by H11 isolated from an alkaline soda lime. H11 was identified as an alkalohalophilic bacterium, and its extracellular serine endoprotease also showed an extreme alkali- and halotolerance. It was remarkably stable in the presence of NaCl up to 5 M. The enzyme was active in a broad range of pH values and temperatures, with an optimum pH of 10.5 and a temperature of 45 °C. It had a molecular mass of about 37 kDa and showed activity against azocasein and a synthetic substrate for the subtilisin-like protease, N-succinyl-l-phenylalanine--nitroanilide. The halo-alkaline protease produced by H11 seems to be significant from an industrial perspective because of its tolerance towards high salinity and alkalinity as well as its stability against some organic solvents, surfactants and oxidants. These properties make the protease suitable for applications in food, detergent and pharmaceutical industries, and also in environmental bioremediation.

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