Insecticidal and Acetylcholinesterase Inhibition Activity of Alkaloidal Extract Against the German Cockroach ()

Overview

Journal J Arthropod Borne Dis

Publisher Tehran University of Medical Sciences

Date 2019 Mar 29

PMID 30918910

Citations 1

Authors

Xianghai Cai

Qingfeng Li

Lei Xiao

Hailiang Lu

Jian Tang

Jianbo Huang

Jianzhong Yuan

Affiliations

Soon will be listed here.

Abstract

Background: (Liliaceae) is perennial medicinal plant widely used to treat various conditions. To determine its insecticidal properties against the German cockroach (), several laboratory tests were carried out.

Methods: A 4kg dry sample of root was purchased from the medicinal material market in Yunnan Province in 2015, China. In contact toxicity tests, alkaloidal extract was topically applied to the abdomen of cockroaches using a micro-applicator. In vitro acetylcholinesterase (AChE) activity tests were performed using a modified Ellman method.

Results: alkaloidal extract was toxic to male adults and 4 nymphs cockroaches, with median lethal dose (LD) values of 14.90μg/insect, 14.21μg/insect for adults and 41.45μg/insect, 39.01μg/insect for 4 nymphs after 24h and 48h exposure, respectively. There was a significant difference between adults and nymphs in terms of tolerance to alkaloidal extract. There was no significant difference in mortalities at 24h and 48h, the lethal effect of alkaloidal extract on German cockroach was quick. AChE activity tests showed that alkaloidal extract had an excellent inhibitory effect on AChE: inhibition in the 4 nymphs and male adults had 50% inhibiting concentration (IC) values of 3.56mg/ml and 5.78mg/ml respectively. The inhibitory effect of AChE activity was positively correlated with inhibitory time (0-20min), at a concentration of 1mg/ml, inhibition of nymph and adult AChE activity had 50% inhibiting time (IT) values of 8.34min and 16.75min, respectively.

Conclusion: may be explored as a potential natural insecticide for control of the German cockroach.

Citing Articles

SARS-CoV-2 Transmission by Arthropod Vectors: A Scoping Review.

Nekoei S, Khamesipour F, Benchimol M, Bueno-Mari R, Ommi D Biomed Res Int. 2022; 2022:4329423.

PMID: 35978635 PMC: 9377902. DOI: 10.1155/2022/4329423.

References

Arruda L, Vailes L, Ferriani V, Santos A, Pomes A, Chapman M . Cockroach allergens and asthma. J Allergy Clin Immunol. 2001; 107(3):419-28. DOI: 10.1067/mai.2001.112854. View

Enan E . Insecticidal activity of essential oils: octopaminergic sites of action. Comp Biochem Physiol C Toxicol Pharmacol. 2001; 130(3):325-37. DOI: 10.1016/s1532-0456(01)00255-1. View

Ellman G, COURTNEY K, ANDRES Jr V, FEATHER-STONE R . A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 1961; 7:88-95. DOI: 10.1016/0006-2952(61)90145-9. View

Omolo M, Okinyo D, Ndiege I, Lwande W, Hassanali A . Repellency of essential oils of some Kenyan plants against Anopheles gambiae. Phytochemistry. 2004; 65(20):2797-802. DOI: 10.1016/j.phytochem.2004.08.035. View

Enan E . Molecular and pharmacological analysis of an octopamine receptor from American cockroach and fruit fly in response to plant essential oils. Arch Insect Biochem Physiol. 2005; 59(3):161-71. DOI: 10.1002/arch.20076. View

Hartmann T . From waste products to ecochemicals: fifty years research of plant secondary metabolism. Phytochemistry. 2007; 68(22-24):2831-46. DOI: 10.1016/j.phytochem.2007.09.017. View

Cong Y, Zhou Y, Chen J, Zeng Y, Wang J . Alkaloid profiling of crude and processed Veratrum nigrum L. through simultaneous determination of ten steroidal alkaloids by HPLC-ELSD. J Pharm Biomed Anal. 2008; 48(3):573-8. DOI: 10.1016/j.jpba.2008.05.023. View

Senthilkumar N, Varma P, Gurusubramanian G . Larvicidal and adulticidal activities of some medicinal plants against the malarial vector, Anopheles stephensi (Liston). Parasitol Res. 2008; 104(2):237-44. DOI: 10.1007/s00436-008-1180-4. View

Phillips A, Appel A, Sims S . Topical toxicity of essential oils to the German cockroach (Dictyoptera: Blattellidae). J Econ Entomol. 2010; 103(2):448-59. DOI: 10.1603/EC09192. View

10.

Phillips A, Appel A . Fumigant toxicity of essential oils to the German cockroach (Dictyoptera: Blattellidae). J Econ Entomol. 2010; 103(3):781-90. DOI: 10.1603/ec09358. View

11.

Kamaraj C, Rahuman A, Mahapatra A, Bagavan A, Elango G . Insecticidal and larvicidal activities of medicinal plant extracts against mosquitoes. Parasitol Res. 2010; 107(6):1337-49. DOI: 10.1007/s00436-010-2006-8. View

12.

Nasirian H . An overview of German cockroach, Blattella germanica, studies conducted in Iran. Pak J Biol Sci. 2011; 13(22):1077-84. DOI: 10.3923/pjbs.2010.1077.1084. View

13.

Yeom H, Kang J, Kim G, Park I . Insecticidal and acetylcholine esterase inhibition activity of Apiaceae plant essential oils and their constituents against adults of German cockroach (Blattella germanica). J Agric Food Chem. 2012; 60(29):7194-203. DOI: 10.1021/jf302009w. View

14.

Morey R, Khandagle A . Bioefficacy of essential oils of medicinal plants against housefly, Musca domestica L. Parasitol Res. 2012; 111(4):1799-805. DOI: 10.1007/s00436-012-3027-2. View

15.

Zhu W, Zhao K, Chu S, Liu Z . Evaluation of Essential Oil and its Three Main Active Ingredients of Chinese Chenopodium ambrosioides (Family: Chenopodiaceae) against Blattella germanica. J Arthropod Borne Dis. 2013; 6(2):90-7. PMC: 3547299. View

16.

Yeom H, Jung C, Kang J, Kim J, Lee J, Kim D . Insecticidal and acetylcholine esterase inhibition activity of Asteraceae plant essential oils and their constituents against adults of the German cockroach (Blattella germanica). J Agric Food Chem. 2015; 63(8):2241-8. DOI: 10.1021/jf505927n. View

17.

Chauhan N, Kumar P, Mishra S, Verma S, Malik A, Sharma S . Insecticidal activity of Jatropha curcas extracts against housefly, Musca domestica. Environ Sci Pollut Res Int. 2015; 22(19):14793-800. DOI: 10.1007/s11356-015-4686-1. View

18.

Sparks T, Nauen R . IRAC: Mode of action classification and insecticide resistance management. Pestic Biochem Physiol. 2015; 121:122-8. DOI: 10.1016/j.pestbp.2014.11.014. View

19.

Liu X, Liu Q, Chen H, Liu Q, Jiang S, Liu Z . Evaluation of Contact Toxicity and Repellency of the Essential Oil of Pogostemon cablin Leaves and Its Constituents Against Blattella germanica (Blattodae: Blattelidae). J Med Entomol. 2015; 52(1):86-92. DOI: 10.1093/jme/tju003. View

20.

Nasirian H . Contamination of cockroaches (Insecta: Blattaria) to medically fungi: A systematic review and meta-analysis. J Mycol Med. 2017; 27(4):427-448. DOI: 10.1016/j.mycmed.2017.04.012. View