A Comparison of Decontamination Methods of Tried-in Preformed Metal Crowns: an In-vivo Study

Overview

Journal Eur Arch Paediatr Dent

Publisher Springer

Specialties Dentistry
Pediatrics

Date 2019 Mar 26

PMID 30906953

Citations 3

Authors

V Darshan

K R Indushekar

B G Saraf

N Sheoran

B Sharma

D Sardana

Affiliations

Soon will be listed here.

Abstract

Aim: To compare the effectiveness of different decontamination methods on tried-in preformed metal crowns (PMCs).

Methods: Sixty unused PMCs and 90 tried-in PMCs from patients were assessed for contamination after culturing for 24 h on liquid media, solid media and differential media for identification of Streptococcus mutans, Staphylococcus aureus, and Escherichia coli. Subsequently, these PMCs were divided equally into the following six groups: autoclave (121 °C, 15 psi for 15 min), 5% sodium hypochlorite (5 min), 5% glutaraldehyde (5 min), 70% isopropyl alcohol (1 min) and normal saline (5 min). The contamination was reassessed, and the Log counts were compared to the pre-decontamination levels using one way ANOVA and paired t-test at a significance level of p < 0.05.

Results: The mean percentage reduction in colony counts was significantly more in the autoclave group compared to glutaraldehyde or sodium hypochlorite groups, glass bead, isopropyl alcohol, and normal saline in this decreasing order.

Conclusions: PMCs supplied by the manufacturer were found to be sterile and can be used directly on patients. The autoclave was the best method of sterilisation, although it did not eliminate the microbes 100%; followed by 5% glutaraldehyde and 5% sodium hypochlorite.

Citing Articles

Comparative Evaluation of Preformed Stainless Steel Crown's Crazing at Different Autoclave Cycles.

Sowkarthicaa P, Mathian V, Gawthaman M, Vinodh S, Manoharan M, Kamatchi M Int J Clin Pediatr Dent. 2023; 16(Suppl 1):S77-S80.

PMID: 37663221 PMC: 10474392. DOI: 10.5005/jp-journals-10005-2608.

Effect of hydrochloric acid, sodium hypochlorite, and autoclave sterilization on the force characteristics of orthodontic NiTi-closed coils.

Noorollahian S, Khaleghi A Dent Res J (Isfahan). 2023; 20:8.

PMID: 36820142 PMC: 9937939.

Decontamination practices of paediatric crowns: a systematic review.

Hogerheyde T, Walsh L, Zafar S Eur Arch Paediatr Dent. 2022; 23(4):527-535.

PMID: 35608756 PMC: 9338143. DOI: 10.1007/s40368-022-00714-w.

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