Genetic Analysis of Myxococcus Xanthus and Isolation of Gene Replacements After Transduction Under Conditions of Limited Homology

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1986 Aug 1

PMID 3090023

Citations 8

Authors

K A OConnor

D R Zusman

Affiliations

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Abstract

Genetic analysis of Myxococcus xanthus is greatly facilitated by the ability to introduce cloned DNA into M. xanthus to generate gene replacement and merodiploid strains. However, gene replacement strains are difficult to obtain when the region(s) of homology between the cloned DNA and the M. xanthus chromosome is limited (less than 1 kilobase). We found that gene replacements can be obtained at an increased frequency by a two-step procedure involving the use of bacteriophage P1 to isolate merodiploid strains followed by generalized transduction to another M. xanthus strain by using phage Mx4.

Citing Articles

Myxococcus xanthus chemotaxis homologs DifD and DifG negatively regulate fibril polysaccharide production.

Black W, Yang Z J Bacteriol. 2004; 186(4):1001-8.

PMID: 14761994 PMC: 344214. DOI: 10.1128/JB.186.4.1001-1008.2004.

Myxococcus xanthus dif genes are required for biogenesis of cell surface fibrils essential for social gliding motility.

Yang Z, Ma X, Tong L, Kaplan H, Shimkets L, Shi W J Bacteriol. 2000; 182(20):5793-8.

PMID: 11004179 PMC: 94702. DOI: 10.1128/JB.182.20.5793-5798.2000.

Methylation of FrzCD defines a discrete step in the developmental program of Myxococcus xanthus.

Geng Y, Yang Z, Downard J, Zusman D, Shi W J Bacteriol. 1998; 180(21):5765-8.

PMID: 9791131 PMC: 107640. DOI: 10.1128/JB.180.21.5765-5768.1998.

A DnaK homolog in Myxococcus xanthus is involved in social motility and fruiting body formation.

Yang Z, Geng Y, Shi W J Bacteriol. 1998; 180(2):218-24.

PMID: 9440508 PMC: 106874. DOI: 10.1128/JB.180.2.218-224.1998.

Identification and localization of the Tgl protein, which is required for Myxococcus xanthus social motility.

Kaiser D J Bacteriol. 1997; 179(13):4372-81.

PMID: 9209056 PMC: 179262. DOI: 10.1128/jb.179.13.4372-4381.1997.

References

Torti S, Zusman D . Genetic characterization of aggregation-defective developmental mutants of Myxococcus xanthus. J Bacteriol. 1981; 147(3):768-75. PMC: 216112. DOI: 10.1128/jb.147.3.768-775.1981. View

Blackhart B, Zusman D . Cloning and complementation analysis of the "Frizzy" genes of Myxococcus xanthus. Mol Gen Genet. 1985; 198(2):243-54. DOI: 10.1007/BF00383002. View

Dworkin M, Kaiser D . Cell interactions in myxobacterial growth and development. Science. 1985; 230(4721):18-24. DOI: 10.1126/science.3929384. View

OConnor K, Zusman D . Coliphage P1-mediated transduction of cloned DNA from Escherichia coli to Myxococcus xanthus: use for complementation and recombinational analyses. J Bacteriol. 1983; 155(1):317-29. PMC: 217683. DOI: 10.1128/jb.155.1.317-329.1983. View

Campos J, GEISSELSODER J, Zusman D . Isolation of bacteriophage MX4, a generalized transducing phage for Myxococcus xanthus. J Mol Biol. 1978; 119(2):167-78. DOI: 10.1016/0022-2836(78)90431-x. View

Morrison C, Zusman D . Myxococcus xanthus mutants with temperature-sensitive, stage-specific defects: evidence for independent pathways in development. J Bacteriol. 1979; 140(3):1036-42. PMC: 216750. DOI: 10.1128/jb.140.3.1036-1042.1979. View

Ikeda H, TOMIZAWA J . Transducing fragments in generalized transduction by phage P1. I. Molecular origin of the fragments. J Mol Biol. 1965; 14(1):85-109. DOI: 10.1016/s0022-2836(65)80232-7. View

GEISSELSODER J, Campos J, Zusman D . Physical characterization of bacteriophage MX4, a generalized transducing phage for Myxococcus xanthus. J Mol Biol. 1978; 119(2):179-89. DOI: 10.1016/0022-2836(78)90432-1. View

Shimkets L, GILL R, Kaiser D . Developmental cell interactions in Myxococcus xanthus and the spoC locus. Proc Natl Acad Sci U S A. 1983; 80(5):1406-10. PMC: 393606. DOI: 10.1073/pnas.80.5.1406. View

10.

Martin S, Sodergren E, Masuda T, Kaiser D . Systematic isolation of transducing phages for Myxococcus xanthus. Virology. 1978; 88(1):44-53. DOI: 10.1016/0042-6822(78)90108-3. View

11.

Bolivar F, Rodriguez R, Greene P, Betlach M, Heyneker H, Boyer H . Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977; 2(2):95-113. View

12.

Zusman D . "Frizzy" mutants: a new class of aggregation-defective developmental mutants of Myxococcus xanthus. J Bacteriol. 1982; 150(3):1430-7. PMC: 216370. DOI: 10.1128/jb.150.3.1430-1437.1982. View

13.

Bachmann B . Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev. 1972; 36(4):525-57. PMC: 408331. DOI: 10.1128/br.36.4.525-557.1972. View

14.

Grunstein M, Hogness D . Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc Natl Acad Sci U S A. 1975; 72(10):3961-5. PMC: 433117. DOI: 10.1073/pnas.72.10.3961. View

15.

Kaiser D, Dworkin M . Gene transfer to myxobacterium by Escherichia coli phage P1. Science. 1975; 187(4177):653-4. DOI: 10.1126/science.803710. View