Baicalin Suppresses Lung Cancer Growth by Targeting PDZ-binding Kinase/T-LAK Cell-originated Protein Kinase

Overview

Journal Biosci Rep

Specialty Cell Biology

Date 2019 Mar 23

PMID 30898980

Citations 13

Authors

Xin Diao

Danfen Yang

Yu Chen

Wentian Liu

Affiliations

Soon will be listed here.

Abstract

Baicalin is the main bioactive component extracted from the traditional Chinese medicine Baical Skullcap Root, and its anti-tumor activity has been studied in previous studies. PDZ-binding kinase/T-LAK cell-originated protein kinase (PBK/TOPK), a serine/threonine protein kinase, is highly expressed in many cancer cells and stimulates the tumorigenic properties, and so, it is a pivotal target for agent to cure cancers. We reported for the first time that baicalin suppressed PBK/TOPK activities by directly binding with PBK/TOPK and studies showed that baicalin suppressed PBK/TOPK activity in JB6 Cl41 cells and H441 lung cancer cells. Moreover, knockdown of PBK/TOPK in H441 cells decreased their sensitivity to baicalin. study indicated that injection of baicalin in H441 tumor-bearing mice effectively suppressed cancer growth. The PBK/TOPK downstream signaling molecules Histone H3 and ERK2 in tumor tissues were also decreased after baicalin treatment. Taken together, baicalin can inhibit proliferation of lung cancer cells as a PBK/TOPK inhibitor both and .

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References

Qian Y, Chen Y, Wang L, Tou J . Effects of baicalin on inflammatory reaction, oxidative stress and PKDl and NF-kB protein expressions in rats with severe acute pancreatitis1. Acta Cir Bras. 2018; 33(7):556-564. DOI: 10.1590/s0102-865020180070000001. View

You J, Cheng J, Yu B, Duan C, Peng J . Baicalin, a Chinese Herbal Medicine, Inhibits the Proliferation and Migration of Human Non-Small Cell Lung Carcinoma (NSCLC) Cells, A549 and H1299, by Activating the SIRT1/AMPK Signaling Pathway. Med Sci Monit. 2018; 24:2126-2133. PMC: 5909419. DOI: 10.12659/msm.909627. View

Seol M, Park J, Jeong J, Lyu J, Han S, Oh S . Role of TOPK in lipopolysaccharide-induced breast cancer cell migration and invasion. Oncotarget. 2017; 8(25):40190-40203. PMC: 5522254. DOI: 10.18632/oncotarget.15360. View

Oh S, Zhu F, Cho Y, Lee K, Kang B, Kim H . T-lymphokine-activated killer cell-originated protein kinase functions as a positive regulator of c-Jun-NH2-kinase 1 signaling and H-Ras-induced cell transformation. Cancer Res. 2007; 67(11):5186-94. DOI: 10.1158/0008-5472.CAN-06-4506. View

Jackman D, Pao W, Riely G, Engelman J, Kris M, Janne P . Clinical definition of acquired resistance to epidermal growth factor receptor tyrosine kinase inhibitors in non-small-cell lung cancer. J Clin Oncol. 2009; 28(2):357-60. PMC: 3870288. DOI: 10.1200/JCO.2009.24.7049. View

Abe Y, Matsumoto S, Kito K, Ueda N . Cloning and expression of a novel MAPKK-like protein kinase, lymphokine-activated killer T-cell-originated protein kinase, specifically expressed in the testis and activated lymphoid cells. J Biol Chem. 2000; 275(28):21525-31. DOI: 10.1074/jbc.M909629199. View

Kim D, Li Y, Reddy K, Lee M, Kim M, Cho Y . Novel TOPK inhibitor HI-TOPK-032 effectively suppresses colon cancer growth. Cancer Res. 2012; 72(12):3060-8. DOI: 10.1158/0008-5472.CAN-11-3851. View

Wei D, Yeh Y, Hung J, Chou T, Wu Y, Lu P . Overexpression of T-LAK cell-originated protein kinase predicts poor prognosis in patients with stage I lung adenocarcinoma. Cancer Sci. 2011; 103(4):731-8. PMC: 7659243. DOI: 10.1111/j.1349-7006.2011.02197.x. View

Kang S, Liu S, Li H, Wang D, Qi X . Baicalin effects on rats with spinal cord injury by anti-inflammatory and regulating the serum metabolic disorder. J Cell Biochem. 2018; 119(9):7767-7779. DOI: 10.1002/jcb.27136. View

10.

Nakamura A, Osonoi T, Terauchi Y . Relationship between urinary sodium excretion and pioglitazone-induced edema. J Diabetes Investig. 2014; 1(5):208-11. PMC: 4020723. DOI: 10.1111/j.2040-1124.2010.00046.x. View

11.

Visbal A, Williams B, Nichols 3rd F, Marks R, Jett J, Aubry M . Gender differences in non-small-cell lung cancer survival: an analysis of 4,618 patients diagnosed between 1997 and 2002. Ann Thorac Surg. 2004; 78(1):209-15. DOI: 10.1016/j.athoracsur.2003.11.021. View

12.

Zykova T, Zhu F, Vakorina T, Zhang J, Higgins L, Urusova D . T-LAK cell-originated protein kinase (TOPK) phosphorylation of Prx1 at Ser-32 prevents UVB-induced apoptosis in RPMI7951 melanoma cells through the regulation of Prx1 peroxidase activity. J Biol Chem. 2010; 285(38):29138-46. PMC: 2937944. DOI: 10.1074/jbc.M110.135905. View

13.

Wienken C, Baaske P, Rothbauer U, Braun D, Duhr S . Protein-binding assays in biological liquids using microscale thermophoresis. Nat Commun. 2010; 1:100. DOI: 10.1038/ncomms1093. View

14.

Matsumoto S, Abe Y, Fujibuchi T, Takeuchi T, Kito K, Ueda N . Characterization of a MAPKK-like protein kinase TOPK. Biochem Biophys Res Commun. 2004; 325(3):997-1004. DOI: 10.1016/j.bbrc.2004.10.133. View

15.

Chen D, Cao J, Zhu B, Wang Z, Wang T, Tang J . Baicalin Attenuates Joint Pain and Muscle Dysfunction by Inhibiting Muscular Oxidative Stress in an Experimental Osteoarthritis Rat Model. Arch Immunol Ther Exp (Warsz). 2018; 66(6):453-461. DOI: 10.1007/s00005-018-0518-6. View

16.

He F, Yan Q, Fan L, Liu Y, Cui J, Wang J . PBK/TOPK in the differential diagnosis of cholangiocarcinoma from hepatocellular carcinoma and its involvement in prognosis of human cholangiocarcinoma. Hum Pathol. 2009; 41(3):415-24. DOI: 10.1016/j.humpath.2009.05.016. View

17.

Li Y, Yang Z, Li W, Xu S, Wang T, Wang T . TOPK promotes lung cancer resistance to EGFR tyrosine kinase inhibitors by phosphorylating and activating c-Jun. Oncotarget. 2016; 7(6):6748-64. PMC: 4872746. DOI: 10.18632/oncotarget.6826. View

18.

Wang T, Zhang Q, Zhang Y, Kang J . Screening of protein kinase inhibitors in natural extracts by capillary electrophoresis combined with liquid chromatography-tandem mass spectrometry. J Chromatogr A. 2014; 1337:188-93. DOI: 10.1016/j.chroma.2014.02.039. View

19.

Shih M, Chen J, Wu Y, Jan Y, Yang B, Lu P . TOPK/PBK promotes cell migration via modulation of the PI3K/PTEN/AKT pathway and is associated with poor prognosis in lung cancer. Oncogene. 2011; 31(19):2389-400. DOI: 10.1038/onc.2011.419. View

20.

Ayllon V, OConnor R . PBK/TOPK promotes tumour cell proliferation through p38 MAPK activity and regulation of the DNA damage response. Oncogene. 2006; 26(24):3451-61. DOI: 10.1038/sj.onc.1210142. View