Expression of /sclerostin in Compressed Periodontal Ligament Cells

Overview

Journal J Dent Sci

Specialty Dentistry

Date 2019 Mar 22

PMID 30894984

Citations 10

Authors

Masae Ueda

Kayoko N Kuroishi

Kaori K Gunjigake

Erina Ikeda

Tatsuo Kawamoto

Affiliations

Soon will be listed here.

Abstract

Background/purpose: Bone resorption and inhibition of bone formation occur on the compressed side during orthodontic tooth movement. Bone formation inhibitory factors such as sclerostin (encoded by ) are secreted on the compressed side by periodontal ligament (PDL) cells. PDL cells control bone metabolism, and compressed PDL cells inhibit bone formation during orthodontic tooth movement. The aim of this study was to identify the inhibitory factors of bone formation in PDL cells.

Materials And Methods: Changes in expression and subsequent protein release from human PDL (hPDL) cells were assessed using the real-time polymerase chain reaction (PCR), semiquantitative PCR, and immunofluorescence in hPDL cells subjected to centrifugal force (40 and 90). To confirm the effects on bone formation, human alveolar bone-derived osteoblasts (hOBs) were grown with the addition of sclerostin peptide. , a compressive force was applied using the Waldo method in rats, and the distribution of sclerostin in PDL tissues was examined by immunohistochemistry.

Results: expression was downregulated by centrifugation at 90 for 24 hours but upregulated by centrifugation at 40 based on real-time PCR, as was confirmed by immunofluorescence staining. The addition of sclerostin peptide significantly decreased the mineralized area in hOBs. However, slightly weakly sclerostin-positive PDL cells were observed on the compressed side .

Conclusion: These results indicate that PDL cells subjected to light compressive force exhibit increased expression of /sclerostin, which inhibits bone formation on the compressed side during orthodontic tooth movement.

Citing Articles

The effect of light compressive and tensile mechanical forces on SOST and POSTN expressions in human periodontal ligament cells: an in vitro study.

Sharifi N, Ahmad Akhoundi M, Hodjat M, Haghighipour N, Veysari S Odontology. 2023; 112(1):91-99.

PMID: 37166745 DOI: 10.1007/s10266-023-00812-1.

The Effect of Sclerostin and Monoclonal Sclerostin Antibody Romosozumab on Osteogenesis and Osteoclastogenesis Mediated by Periodontal Ligament Fibroblasts.

Pigeaud K, Rietveld M, Witvliet A, Hogervorst J, Zhang C, Forouzanfar T Int J Mol Sci. 2023; 24(8).

PMID: 37108735 PMC: 10145870. DOI: 10.3390/ijms24087574.

regulates expression during maintenance of periodontal homeostasis.

Yoshikazu Manabe , Shiga M, Kometani-Gunjigake K, Nakao-Kuroishi K, Mizuhara M, Toyono T J Dent Sci. 2022; 17(4):1714-1721.

PMID: 36299324 PMC: 9588790. DOI: 10.1016/j.jds.2022.02.015.

Dynamic alternations of RANKL/OPG ratio expressed by cementocytes in response to orthodontic‑induced external apical root resorption in a rat model.

Wei T, Shan Z, Wen X, Zhao N, Shen G Mol Med Rep. 2022; 26(1).

PMID: 35593309 PMC: 9178691. DOI: 10.3892/mmr.2022.12744.

Histomorphometric assessment of implant coated with mixture of nano-alumina and fluorapatite in rabbits.

Ibrahim S, Rafeeq A, Ahmedhamdi M Saudi Dent J. 2021; 33(8):1142-1148.

PMID: 34938060 PMC: 8665202. DOI: 10.1016/j.sdentj.2021.02.005.

References

Vandevska-Radunovic V . Neural modulation of inflammatory reactions in dental tissues incident to orthodontic tooth movement. A review of the literature. Eur J Orthod. 1999; 21(3):231-47. DOI: 10.1093/ejo/21.3.231. View

Melsen B . Tissue reaction to orthodontic tooth movement--a new paradigm. Eur J Orthod. 2002; 23(6):671-81. DOI: 10.1093/ejo/23.6.671. View

Redlich M, Asher Roos H, Reichenberg E, Zaks B, Mussig D, Baumert U . Expression of tropoelastin in human periodontal ligament fibroblasts after simulation of orthodontic force. Arch Oral Biol. 2003; 49(2):119-24. DOI: 10.1016/j.archoralbio.2003.08.002. View

Baumert U, Golan I, Becker B, Hrala B, Redlich M, Roos H . Pressure simulation of orthodontic force in osteoblasts: a pilot study. Orthod Craniofac Res. 2004; 7(1):3-9. DOI: 10.1046/j.1601-6335.2003.00270.x. View

Ren Y, Maltha J, Kuijpers-Jagtman A . The rat as a model for orthodontic tooth movement--a critical review and a proposed solution. Eur J Orthod. 2004; 26(5):483-90. DOI: 10.1093/ejo/26.5.483. View

Li X, Zhang Y, Kang H, Liu W, Liu P, Zhang J . Sclerostin binds to LRP5/6 and antagonizes canonical Wnt signaling. J Biol Chem. 2005; 280(20):19883-7. DOI: 10.1074/jbc.M413274200. View

Krishnan V, Davidovitch Z . Cellular, molecular, and tissue-level reactions to orthodontic force. Am J Orthod Dentofacial Orthop. 2006; 129(4):469.e1-32. DOI: 10.1016/j.ajodo.2005.10.007. View

Bodine P, Komm B . Wnt signaling and osteoblastogenesis. Rev Endocr Metab Disord. 2006; 7(1-2):33-9. DOI: 10.1007/s11154-006-9002-4. View

van Bezooijen R, DeRuiter M, Vilain N, Monteiro R, Visser A, van der Wee-Pals L . SOST expression is restricted to the great arteries during embryonic and neonatal cardiovascular development. Dev Dyn. 2006; 236(2):606-12. DOI: 10.1002/dvdy.21054. View

10.

Davidovitch Z . Tooth movement. Crit Rev Oral Biol Med. 1991; 2(4):411-50. DOI: 10.1177/10454411910020040101. View

11.

Yavropoulou M, Yovos J . The role of the Wnt signaling pathway in osteoblast commitment and differentiation. Hormones (Athens). 2007; 6(4):279-94. DOI: 10.14310/horm.2002.1111024. View

12.

Robling A, Niziolek P, Baldridge L, Condon K, Allen M, Alam I . Mechanical stimulation of bone in vivo reduces osteocyte expression of Sost/sclerostin. J Biol Chem. 2007; 283(9):5866-75. DOI: 10.1074/jbc.M705092200. View

13.

Piters E, Boudin E, Van Hul W . Wnt signaling: a win for bone. Arch Biochem Biophys. 2008; 473(2):112-6. DOI: 10.1016/j.abb.2008.03.006. View

14.

Zhao Y, Wang C, Li S, Song H, Wei F, Pan K . Expression of Osterix in mechanical stress-induced osteogenic differentiation of periodontal ligament cells in vitro. Eur J Oral Sci. 2008; 116(3):199-206. DOI: 10.1111/j.1600-0722.2008.00533.x. View

15.

Veverka V, Henry A, Slocombe P, Ventom A, Mulloy B, Muskett F . Characterization of the structural features and interactions of sclerostin: molecular insight into a key regulator of Wnt-mediated bone formation. J Biol Chem. 2009; 284(16):10890-900. PMC: 2667775. DOI: 10.1074/jbc.M807994200. View

16.

Kubota T, Michigami T, Ozono K . Wnt signaling in bone metabolism. J Bone Miner Metab. 2009; 27(3):265-71. DOI: 10.1007/s00774-009-0064-8. View

17.

Jager A, Gotz W, Lossdorfer S, Rath-Deschner B . Localization of SOST/sclerostin in cementocytes in vivo and in mineralizing periodontal ligament cells in vitro. J Periodontal Res. 2009; 45(2):246-54. DOI: 10.1111/j.1600-0765.2009.01227.x. View

18.

Gunjigake K, Goto T, Nakao K, Kobayashi S, Yamaguchi K . Activation of satellite glial cells in rat trigeminal ganglion after upper molar extraction. Acta Histochem Cytochem. 2009; 42(5):143-9. PMC: 2775105. DOI: 10.1267/ahc.09017. View

19.

Tu X, Rhee Y, Condon K, Bivi N, Allen M, Dwyer D . Sost downregulation and local Wnt signaling are required for the osteogenic response to mechanical loading. Bone. 2011; 50(1):209-17. PMC: 3246572. DOI: 10.1016/j.bone.2011.10.025. View

20.

Liao W, Okada M, Inami K, Hashimoto Y, Matsumoto N . Cell survival and gene expression under compressive stress in a three-dimensional in vitro human periodontal ligament-like tissue model. Cytotechnology. 2014; 68(2):249-60. PMC: 4754250. DOI: 10.1007/s10616-014-9775-3. View