Similarities and Differences in Tissue Distribution of DLK1 and DLK2 During E16.5 Mouse Embryogenesis

Overview

Journal Histochem Cell Biol

Publisher Springer

Specialties Biochemistry
Cell Biology

Date 2019 Mar 20

PMID 30888503

Citations 3

Authors

P Garcia-Gallastegi

A Ruiz-Garcia

G Ibarretxe

S Rivero-Hinojosa

A D Gonzalez-Siccha

J Laborda

O Crende

F Unda

J J Garcia-Ramirez

Affiliations

Soon will be listed here.

Abstract

DLK1 and DLK2 are transmembrane proteins belonging to the EGF-like repeat-containing family that function as non-canonical NOTCH inhibitory ligands. DLK1 is usually downregulated after embryo development and its distribution in some adult and embryonic tissues has been described. However, the expression and role of DLK2 in embryo and adult tissues remains unclear. To better understand the relevance of both proteins during embryo development, we analyzed the expression pattern of DLK1 and DLK2 in 16.5-day-old mouse embryos (E16.5) and evaluated the possible relationship between these two proteins in embryo tissues and cell types. We found that DLK1 and DLK2 proteins exhibited a broad distribution pattern, which was detected in developing mouse organs from each of the three germ layers: ectoderm (brain, salivary glands), mesoderm (skeletal muscle, vertebral column, kidney, cartilage), and endoderm (thymus, lung, pancreas, intestine, liver). The expression pattern of DLK1 and DLK2 indicates that both proteins could play a synergistic role during cell differentiation. This study provides additional information for understanding temporal and site-specific effects of DLK1 and DLK2 during embryo morphogenesis and cell differentiation.

Citing Articles

Dlk2 interacts with Syap1 to activate Akt signaling pathway during osteoclast formation.

Chen X, Chen X, Chao R, Wang Y, Mao Y, Fan B Cell Death Dis. 2023; 14(9):589.

PMID: 37669921 PMC: 10480461. DOI: 10.1038/s41419-023-06107-1.

Disruption of neuronal RHEB signaling impairs oligodendrocyte differentiation and myelination through mTORC1-DLK1 axis.

Huang H, Jing B, Zhu F, Jiang W, Tang P, Shi L Cell Rep. 2023; 42(7):112801.

PMID: 37463107 PMC: 11849431. DOI: 10.1016/j.celrep.2023.112801.

DLK2 Acts as a Potential Prognostic Biomarker for Clear Cell Renal Cell Carcinoma Based on Bioinformatics Analysis.

Lee M, Lee Y, Huang S, Chang C, Ko C, Lee W Genes (Basel). 2022; 13(4).

PMID: 35456435 PMC: 9030291. DOI: 10.3390/genes13040629.

Cell signaling regulation in salivary gland development.

Suzuki A, Ogata K, Iwata J Cell Mol Life Sci. 2021; 78(7):3299-3315.

PMID: 33449148 PMC: 11071883. DOI: 10.1007/s00018-020-03741-2.

References

Gubina E, Ruiz-Hidalgo M, Baladron V, Laborda J . Assignment of DLK1 to human chromosome band 14q32 by in situ hybridization. Cytogenet Cell Genet. 1999; 84(3-4):206-7. DOI: 10.1159/000015259. View

Floridon C, Jensen C, Thorsen P, Nielsen O, Sunde L, Westergaard J . Does fetal antigen 1 (FA1) identify cells with regenerative, endocrine and neuroendocrine potentials? A study of FA1 in embryonic, fetal, and placental tissue and in maternal circulation. Differentiation. 2000; 66(1):49-59. DOI: 10.1046/j.1432-0436.2000.066001049.x. View

Bamman M, Shipp J, Jiang J, Gower B, Hunter G, Goodman A . Mechanical load increases muscle IGF-I and androgen receptor mRNA concentrations in humans. Am J Physiol Endocrinol Metab. 2001; 280(3):E383-90. DOI: 10.1152/ajpendo.2001.280.3.E383. View

Tanimizu N, Nishikawa M, Saito H, Tsujimura T, Miyajima A . Isolation of hepatoblasts based on the expression of Dlk/Pref-1. J Cell Sci. 2003; 116(Pt 9):1775-86. DOI: 10.1242/jcs.00388. View

Pawson T, Nash P . Assembly of cell regulatory systems through protein interaction domains. Science. 2003; 300(5618):445-52. DOI: 10.1126/science.1083653. View

Abdallah B, Jensen C, Gutierrez G, Leslie R, Jensen T, Kassem M . Regulation of human skeletal stem cells differentiation by Dlk1/Pref-1. J Bone Miner Res. 2004; 19(5):841-52. DOI: 10.1359/JBMR.040118. View

Davis E, Jensen C, Schroder H, Farnir F, Shay-Hadfield T, Kliem A . Ectopic expression of DLK1 protein in skeletal muscle of padumnal heterozygotes causes the callipyge phenotype. Curr Biol. 2004; 14(20):1858-62. DOI: 10.1016/j.cub.2004.09.079. View

Baladron V, Ruiz-Hidalgo M, Nueda M, Diaz-Guerra M, Garcia-Ramirez J, Bonvini E . dlk acts as a negative regulator of Notch1 activation through interactions with specific EGF-like repeats. Exp Cell Res. 2005; 303(2):343-59. DOI: 10.1016/j.yexcr.2004.10.001. View

Tremblay K, Zaret K . Distinct populations of endoderm cells converge to generate the embryonic liver bud and ventral foregut tissues. Dev Biol. 2005; 280(1):87-99. DOI: 10.1016/j.ydbio.2005.01.003. View

10.

Wang J, Shaner N, Mittal B, Zhou Q, Chen J, Sanger J . Dynamics of Z-band based proteins in developing skeletal muscle cells. Cell Motil Cytoskeleton. 2005; 61(1):34-48. PMC: 1993831. DOI: 10.1002/cm.20063. View

11.

Yevtodiyenko A, Schmidt J . Dlk1 expression marks developing endothelium and sites of branching morphogenesis in the mouse embryo and placenta. Dev Dyn. 2006; 235(4):1115-23. DOI: 10.1002/dvdy.20705. View

12.

Wang Y, Sul H . Ectodomain shedding of preadipocyte factor 1 (Pref-1) by tumor necrosis factor alpha converting enzyme (TACE) and inhibition of adipocyte differentiation. Mol Cell Biol. 2006; 26(14):5421-35. PMC: 1592724. DOI: 10.1128/MCB.02437-05. View

13.

Nueda M, Baladron V, Garcia-Ramirez J, Sanchez-Solana B, Ruvira M, Rivero S . The novel gene EGFL9/Dlk2, highly homologous to Dlk1, functions as a modulator of adipogenesis. J Mol Biol. 2007; 367(5):1270-80. DOI: 10.1016/j.jmb.2006.10.020. View

14.

Nueda M, Baladron V, Sanchez-Solana B, Ballesteros M, Laborda J . The EGF-like protein dlk1 inhibits notch signaling and potentiates adipogenesis of mesenchymal cells. J Mol Biol. 2007; 367(5):1281-93. DOI: 10.1016/j.jmb.2006.10.043. View

15.

Tucker A . Salivary gland development. Semin Cell Dev Biol. 2007; 18(2):237-44. DOI: 10.1016/j.semcdb.2007.01.006. View

16.

Ansell P, Zhou Y, Schjeide B, Kerner A, Zhao J, Zhang X . Regulation of growth hormone expression by Delta-like protein 1 (Dlk1). Mol Cell Endocrinol. 2007; 271(1-2):55-63. PMC: 1974851. DOI: 10.1016/j.mce.2007.04.002. View

17.

Taylor M, Yeager K, Morrison S . Physiological Notch signaling promotes gliogenesis in the developing peripheral and central nervous systems. Development. 2007; 134(13):2435-47. PMC: 2653864. DOI: 10.1242/dev.005520. View

18.

Hermida C, Garces C, de Oya M, Cano B, Martinez-Costa O, Rivero S . The serum levels of the EGF-like homeotic protein dlk1 correlate with different metabolic parameters in two hormonally different children populations in Spain. Clin Endocrinol (Oxf). 2008; 69(2):216-24. DOI: 10.1111/j.1365-2265.2008.03170.x. View

19.

Nueda M, Garcia-Ramirez J, Laborda J, Baladron V . dlk1 specifically interacts with insulin-like growth factor binding protein 1 to modulate adipogenesis of 3T3-L1 cells. J Mol Biol. 2008; 379(3):428-42. DOI: 10.1016/j.jmb.2008.03.070. View

20.

Raghunandan R, Ruiz-Hidalgo M, Jia Y, Ettinger R, Rudikoff E, Riggins P . Dlk1 influences differentiation and function of B lymphocytes. Stem Cells Dev. 2008; 17(3):495-507. PMC: 3189718. DOI: 10.1089/scd.2007.0102. View