Luminescent CeO:Eu Nanocrystals for Robust in Situ HO Real-time Detection in Bacterial Cell Cultures

Overview

Journal Biosens Bioelectron

Specialties Biochemistry
Biotechnology

Date 2019 Mar 19

PMID 30884315

Citations 7

Authors

Dorian F Henning

Padryk Merkl

Changhun Yun

Federico Iovino

Ling Xie

Eleftherios Mouzourakis

Constantinos Moularas

Yiannis Deligiannakis

Birgitta Henriques-Normark

Klaus Leifer

Georgios A Sotiriou

Affiliations

Soon will be listed here.

Abstract

Hydrogen peroxide (HO) quantification in biomedicine is valuable as inflammation biomarker but also in assays employing enzymes that generate or consume HO linked to a specific biomarker. Optical HO detection is typically performed through peroxidase-coupled reactions utilizing organic dyes that suffer, however, from poor stability/reproducibility and also cannot be employed in situ in dynamic complex cell cultures to monitor HO levels in real-time. Here, we utilize enzyme-mimetic CeO nanocrystals that are sensitive to HO and study the effect of HO presence on their electronic and luminescent properties. We produce and dope with Eu these particles in a single-step by flame synthesis and directly deposit them on Si and glass substrates to fabricate nanoparticle layers to monitor in real-time and in situ the HO concentrations generated by Streptococcus pneumoniae clinical isolates. Furthermore, the small CeO:Eu nanocrystals are combined in a single-step with larger, non-responsive YO:Tb nanoparticles during their double-nozzle flame synthesis to engineer hybrid luminescent nanoaggregates as ratiometric robust biosensors. We demonstrate the functionality of these biosensors by monitoring their response in the presence of a broad range of HO concentrations in vitro from S. pneumoniae, highlighting their potential for facile real-time HO detection in vitro in cell cultures.

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References

Lee S, Song W, Cho M, Puppala H, Nguyen P, Zhu H . Antioxidant properties of cerium oxide nanocrystals as a function of nanocrystal diameter and surface coating. ACS Nano. 2013; 7(11):9693-703. DOI: 10.1021/nn4026806. View

Henriques-Normark B, Tuomanen E . The pneumococcus: epidemiology, microbiology, and pathogenesis. Cold Spring Harb Perspect Med. 2013; 3(7). PMC: 3685878. DOI: 10.1101/cshperspect.a010215. View

Zeng H, Zhang L, Rong L, Liang R, Qiu J . A luminescent lanthanide coordination polymer based on energy transfer from metal to metal for hydrogen peroxide detection. Biosens Bioelectron. 2016; 89(Pt 2):721-727. DOI: 10.1016/j.bios.2016.11.020. View

Wei H, Wang E . Nanomaterials with enzyme-like characteristics (nanozymes): next-generation artificial enzymes. Chem Soc Rev. 2013; 42(14):6060-93. DOI: 10.1039/c3cs35486e. View

Yang L, Li N, Pan W, Yu Z, Tang B . Real-time imaging of mitochondrial hydrogen peroxide and pH fluctuations in living cells using a fluorescent nanosensor. Anal Chem. 2015; 87(7):3678-84. DOI: 10.1021/ac503975x. View

Cafun J, Kvashnina K, Casals E, Puntes V, Glatzel P . Absence of Ce3+ sites in chemically active colloidal ceria nanoparticles. ACS Nano. 2013; 7(12):10726-32. DOI: 10.1021/nn403542p. View

Halliwell B, Clement M, Long L . Hydrogen peroxide in the human body. FEBS Lett. 2000; 486(1):10-3. DOI: 10.1016/s0014-5793(00)02197-9. View

Liu J, Liu Y, Liu Q, Li C, Sun L, Li F . Iridium(III) complex-coated nanosystem for ratiometric upconversion luminescence bioimaging of cyanide anions. J Am Chem Soc. 2011; 133(39):15276-9. DOI: 10.1021/ja205907y. View

Li Y, He X, Yin J, Ma Y, Zhang P, Li J . Acquired superoxide-scavenging ability of ceria nanoparticles. Angew Chem Int Ed Engl. 2014; 54(6):1832-5. DOI: 10.1002/anie.201410398. View

10.

Pratsinis A, Kelesidis G, Zuercher S, Krumeich F, Bolisetty S, Mezzenga R . Enzyme-Mimetic Antioxidant Luminescent Nanoparticles for Highly Sensitive Hydrogen Peroxide Biosensing. ACS Nano. 2017; 11(12):12210-12218. DOI: 10.1021/acsnano.7b05518. View

11.

Sotiriou G, Schneider M, Pratsinis S . Color-tunable nanophosphors by co-doping flame-made YO with Tb and Eu. J Phys Chem C Nanomater Interfaces. 2013; 115(4):1084-1089. PMC: 3667478. DOI: 10.1021/jp106137u. View

12.

Zeng H, Qiu W, Zhang L, Liang R, Qiu J . Lanthanide Coordination Polymer Nanoparticles as an Excellent Artificial Peroxidase for Hydrogen Peroxide Detection. Anal Chem. 2016; 88(12):6342-8. DOI: 10.1021/acs.analchem.6b00630. View

13.

Andisi V, Hinojosa C, de Jong A, Kuipers O, Orihuela C, Bijlsma J . Pneumococcal gene complex involved in resistance to extracellular oxidative stress. Infect Immun. 2012; 80(3):1037-49. PMC: 3294666. DOI: 10.1128/IAI.05563-11. View

14.

Lv C, Di W, Liu Z, Zheng K, Qin W . Luminescent CePO₄:Tb colloids for H₂O₂ and glucose sensing. Analyst. 2014; 139(18):4547-55. DOI: 10.1039/c4an00952e. View

15.

Walkey C, Das S, Seal S, Erlichman J, Heckman K, Ghibelli L . Catalytic Properties and Biomedical Applications of Cerium Oxide Nanoparticles. Environ Sci Nano. 2015; 2(1):33-53. PMC: 4508017. DOI: 10.1039/C4EN00138A. View

16.

Mukherjee S, Sudarsan V, Vatsa R, Godbole S, Kadam R, Bhatta U . Effect of structure, particle size and relative concentration of Eu(3+) and Tb(3+) ions on the luminescence properties of Eu(3+) co-doped Y(2)O(3):Tb nanoparticles. Nanotechnology. 2011; 19(32):325704. DOI: 10.1088/0957-4484/19/32/325704. View

17.

Iovino F, Seinen J, Henriques-Normark B, van Dijl J . How Does Streptococcus pneumoniae Invade the Brain?. Trends Microbiol. 2016; 24(4):307-315. DOI: 10.1016/j.tim.2015.12.012. View

18.

Feng Y, Cheng J, Zhou L, Zhou X, Xiang H . Ratiometric optical oxygen sensing: a review in respect of material design. Analyst. 2012; 137(21):4885-901. DOI: 10.1039/c2an35907c. View

19.

Liu B, Sun Z, Huang P, Liu J . Hydrogen peroxide displacing DNA from nanoceria: mechanism and detection of glucose in serum. J Am Chem Soc. 2015; 137(3):1290-5. DOI: 10.1021/ja511444e. View

20.

Sotiriou G, Schneider M, Pratsinis S . Green, Silica-Coated Monoclinic Y(2)O(3):Tb(3+) Nanophosphors: Flame Synthesis and Characterization. J Phys Chem C Nanomater Interfaces. 2013; 116(7):4493-4499. PMC: 3568749. DOI: 10.1021/jp211722z. View