Alveolar Macrophage Phagocytosis and Bacteria Clearance in Mice

Overview

Journal J Vis Exp

Specialties Biology
General Medicine

Date 2019 Mar 19

PMID 30882784

Citations 14

Authors

Nagaraja Nagre

Xiaofei Cong

Andrew C Pearson

Xiaoli Zhao

Affiliations

Soon will be listed here.

Abstract

Alveolar macrophages (AMs) guard the alveolar space of the lung. Phagocytosis by AMs plays a critical role in the defense against invading pathogens, the removal of dead cells or foreign particles, and in the resolution of inflammatory responses and tissue remodeling, processes that are mediated by various surface receptors of the AMs. Here, we report methods for the analysis of the phagocytic function of AMs using in vitro and in vivo assays and experimental strategies to differentiate between the pattern recognition receptor-, complement receptor-, and Fc gamma receptor-mediated phagocytosis. Finally, we discuss a method to establish and characterize a P. aeruginosa pneumonia model in mice to assess bacterial clearance in vivo. These assays represent the most common methods to evaluate AM functions and can also be used to study macrophage function and bacterial clearance in other organs.

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