Cerium Oxide Nanoparticles Regulate Insulin Sensitivity and Oxidative Markers in 3T3-L1 Adipocytes and C2C12 Myotubes

Overview

Journal Oxid Med Cell Longev

Publisher Wiley

Specialties Cell Biology
Endocrinology

Date 2019 Mar 14

PMID 30863477

Citations 5

Authors

Amaya Lopez-Pascual

Andoni Urrutia-Sarratea

Silvia Lorente-Cebrian

J Alfredo Martinez

Pedro Gonzalez-Muniesa

Affiliations

Soon will be listed here.

Abstract

Insulin resistance is associated with oxidative stress, mitochondrial dysfunction, and a chronic low-grade inflammatory status. In this sense, cerium oxide nanoparticles (CeO NPs) are promising nanomaterials with antioxidant and anti-inflammatory properties. Thus, we aimed to evaluate the effect of CeO NPs in mouse 3T3-L1 adipocytes, RAW 264.7 macrophages, and C2C12 myotubes under control or proinflammatory conditions. Macrophages were treated with LPS, and both adipocytes and myotubes with conditioned medium (25% LPS-activated macrophages medium) to promote inflammation. CeO NPs showed a mean size of ≤25.3 nm (96.7%) and a zeta potential of 30.57 ± 0.58 mV, suitable for cell internalization. CeO NPs reduced extracellular reactive oxygen species (ROS) in adipocytes with inflammation while increased in myotubes with control medium. The CeO NPs increased mitochondrial content was observed in adipocytes under proinflammatory conditions. Furthermore, the expression of and increased in adipocytes treated with CeO NPs. In myotubes, both and were downregulated while was upregulated. Overall, our results suggest that CeO NPs could potentially have an insulin-sensitizing effect specifically on adipose tissue and skeletal muscle. However, further research is needed to confirm these findings.

Citing Articles

Management of cardiovascular disease by cerium oxide nanoparticles via alleviating oxidative stress and adipokine abnormalities.

Bashandy S, Elbaset M, Ibrahim F, Abdelrahman S, Moussa S, El-Seidy A Sci Rep. 2025; 15(1):5709.

PMID: 39962072 PMC: 11833101. DOI: 10.1038/s41598-025-85794-6.

Effect of cerium oxide on iron metabolism in mice.

Kanome Y, Ohtomo S, Nakatsu M, Kohno M, Fukui K J Clin Biochem Nutr. 2024; 75(3):190-196.

PMID: 39583972 PMC: 11579851. DOI: 10.3164/jcbn.24-38.

Early Pregnancy Exposure to Rare Earth Elements and Risk of Gestational Diabetes Mellitus: A Nested Case-Control Study.

Xu X, Wang Y, Han N, Yang X, Ji Y, Liu J Front Endocrinol (Lausanne). 2022; 12:774142.

PMID: 34987477 PMC: 8721846. DOI: 10.3389/fendo.2021.774142.

Protective effects of cerium oxide nanoparticles in non-alcoholic fatty liver disease (NAFLD) and carbon tetrachloride-induced liver damage in rats: Study on intestine and liver.

Abbasi E, Vafaei S, Naseri N, Darini A, Azandaryani M, Ara F Metabol Open. 2021; 12:100151.

PMID: 34870139 PMC: 8626579. DOI: 10.1016/j.metop.2021.100151.

Noncytotoxic silver nanoparticles as a new antimicrobial strategy.

Skora B, Krajewska U, Nowak A, Dziedzic A, Barylyak A, Kus-Liskiewicz M Sci Rep. 2021; 11(1):13451.

PMID: 34188097 PMC: 8242066. DOI: 10.1038/s41598-021-92812-w.

References

Pfaffl M . A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001; 29(9):e45. PMC: 55695. DOI: 10.1093/nar/29.9.e45. View

Evans J, Goldfine I, Maddux B, Grodsky G . Are oxidative stress-activated signaling pathways mediators of insulin resistance and beta-cell dysfunction?. Diabetes. 2002; 52(1):1-8. DOI: 10.2337/diabetes.52.1.1. View

Petersen K, Shulman G . Etiology of insulin resistance. Am J Med. 2006; 119(5 Suppl 1):S10-6. PMC: 2995525. DOI: 10.1016/j.amjmed.2006.01.009. View

Kahn S, Hull R, Utzschneider K . Mechanisms linking obesity to insulin resistance and type 2 diabetes. Nature. 2006; 444(7121):840-6. DOI: 10.1038/nature05482. View

Niu J, Azfer A, Rogers L, Wang X, Kolattukudy P . Cardioprotective effects of cerium oxide nanoparticles in a transgenic murine model of cardiomyopathy. Cardiovasc Res. 2007; 73(3):549-59. PMC: 1855085. DOI: 10.1016/j.cardiores.2006.11.031. View

Heckert E, Karakoti A, Seal S, Self W . The role of cerium redox state in the SOD mimetic activity of nanoceria. Biomaterials. 2008; 29(18):2705-9. PMC: 2396488. DOI: 10.1016/j.biomaterials.2008.03.014. View

Hirst S, Karakoti A, Tyler R, Sriranganathan N, Seal S, Reilly C . Anti-inflammatory properties of cerium oxide nanoparticles. Small. 2009; 5(24):2848-56. DOI: 10.1002/smll.200901048. View

Alberti K, Eckel R, Grundy S, Zimmet P, Cleeman J, Donato K . Harmonizing the metabolic syndrome: a joint interim statement of the International Diabetes Federation Task Force on Epidemiology and Prevention; National Heart, Lung, and Blood Institute; American Heart Association; World Heart Federation;.... Circulation. 2009; 120(16):1640-5. DOI: 10.1161/CIRCULATIONAHA.109.192644. View

Patti M, Corvera S . The role of mitochondria in the pathogenesis of type 2 diabetes. Endocr Rev. 2010; 31(3):364-95. PMC: 3365846. DOI: 10.1210/er.2009-0027. View

10.

Rains J, Jain S . Oxidative stress, insulin signaling, and diabetes. Free Radic Biol Med. 2010; 50(5):567-75. PMC: 3557825. DOI: 10.1016/j.freeradbiomed.2010.12.006. View

11.

Donath M, Shoelson S . Type 2 diabetes as an inflammatory disease. Nat Rev Immunol. 2011; 11(2):98-107. DOI: 10.1038/nri2925. View

12.

Scheller J, Chalaris A, Schmidt-Arras D, Rose-John S . The pro- and anti-inflammatory properties of the cytokine interleukin-6. Biochim Biophys Acta. 2011; 1813(5):878-88. DOI: 10.1016/j.bbamcr.2011.01.034. View

13.

Niu J, Wang K, Kolattukudy P . Cerium oxide nanoparticles inhibit oxidative stress and nuclear factor-κB activation in H9c2 cardiomyocytes exposed to cigarette smoke extract. J Pharmacol Exp Ther. 2011; 338(1):53-61. PMC: 3126650. DOI: 10.1124/jpet.111.179978. View

14.

Hirst S, Karakoti A, Singh S, Self W, Tyler R, Seal S . Bio-distribution and in vivo antioxidant effects of cerium oxide nanoparticles in mice. Environ Toxicol. 2011; 28(2):107-18. DOI: 10.1002/tox.20704. View

15.

Chen L, Magliano D, Zimmet P . The worldwide epidemiology of type 2 diabetes mellitus--present and future perspectives. Nat Rev Endocrinol. 2011; 8(4):228-36. DOI: 10.1038/nrendo.2011.183. View

16.

Yokel R, Au T, MacPhail R, Hardas S, Butterfield D, Sultana R . Distribution, elimination, and biopersistence to 90 days of a systemically introduced 30 nm ceria-engineered nanomaterial in rats. Toxicol Sci. 2012; 127(1):256-68. DOI: 10.1093/toxsci/kfs067. View

17.

Hussain S, Al-Nsour F, Rice A, Marshburn J, Ji Z, Zink J . Cerium dioxide nanoparticles do not modulate the lipopolysaccharide-induced inflammatory response in human monocytes. Int J Nanomedicine. 2012; 7:1387-97. PMC: 3310407. DOI: 10.2147/IJN.S29429. View

18.

Hussain S, Al-Nsour F, Rice A, Marshburn J, Yingling B, Ji Z . Cerium dioxide nanoparticles induce apoptosis and autophagy in human peripheral blood monocytes. ACS Nano. 2012; 6(7):5820-9. PMC: 4582414. DOI: 10.1021/nn302235u. View

19.

Ciofani G, Genchi G, Liakos I, Cappello V, Gemmi M, Athanassiou A . Effects of cerium oxide nanoparticles on PC12 neuronal-like cells: proliferation, differentiation, and dopamine secretion. Pharm Res. 2013; 30(8):2133-45. DOI: 10.1007/s11095-013-1071-y. View

20.

El-Kadre L, Tinoco A . Interleukin-6 and obesity: the crosstalk between intestine, pancreas and liver. Curr Opin Clin Nutr Metab Care. 2013; 16(5):564-8. DOI: 10.1097/MCO.0b013e32836410e6. View