A CDNA Clone Used to Study MRNA Inducible in Human Tonsillar Lymphocytes by a Tumor Promoter

Overview

Journal J Biochem

Publisher Oxford University Press

Specialty Biochemistry

Date 1986 Mar 1

PMID 3086300

Citations 28

Authors

K Obaru

M Fukuda

S Maeda

K Shimada

Affiliations

Soon will be listed here.

Abstract

A cDNA clone inducible by either a tumor promoter, 12-o-tetradecanoyl phorbol-13-acetate (TPA), or a T-cell mitogen, phytohemagglutinin (PHA), was isolated from a cDNA library constructed from the poly(A) + RNAs of TPA- and PHA-stimulated human tonsillar lymphocytes, and was named pLD78. Stimulation of the tonsillar lymphocytes with either TPA or PHA increased the amount of pLD78-specific RNA by about 10-fold, and simultaneous stimulation with TPA and PHA, by at least 30-fold. Analysis of the pLD78 cDNA sequence revealed that it codes for a polypeptide consisting of 92 amino acid residues, including a putative signal sequence. Moreover, the sequence of the 5' flanking region of the nuclear DNA encoding for the pLD78 cDNA showed a significant homology with the corresponding regions of the human interleukin 2 and immune interferon genes.

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