Nucleotide Sequence of the Gene for Cytochrome B558 of the Bacillus Subtilis Succinate Dehydrogenase Complex

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1986 Jun 1

PMID 3086287

Citations 16

Authors

K Magnusson

M K Philips

J R Guest

L Rutberg

Affiliations

Soon will be listed here.

Abstract

The nucleotide sequence was determined for the first part of the Bacillus subtilis sdh operon. An open reading frame corresponding to the structural gene, sdhA, for cytochrome b558 was identified. The predicted molecular weight of the cytochrome (excluding the N-terminal methionine) is 22,770. It is a very hydrophobic protein with five probable membrane-spanning segments. There is little homology between the B. subtilis cytochrome b558 and cytochrome b of mitochondrial complex III from different organisms or between cytochrome b558 and the hydrophobic sdhC and sdhD peptides of the Escherichia coli sdh operon. About 30 bases downstream of the sdhA stop codon, a new open reading frame starts. The nucleotide sequence predicts the presence of a typical flavin-binding peptide which identifies this reading frame as part of the sdhB gene. Seven bases upstream of the sdhA initiation codon ATG there is a typical B. subtilis ribosome binding site (free energy of interaction, -63 kJ), and further upstream, tentative sigma 55 and sigma 32 promoter sequences were found. The upstream region also contains two 12-base-long direct repeats; their significance is unknown.

Citing Articles

Germline mutations in children and adults with cancer.

Dubard Gault M, Mandelker D, DeLair D, Stewart C, Kemel Y, Sheehan M Cold Spring Harb Mol Case Stud. 2018; 4(4).

PMID: 30068732 PMC: 6071569. DOI: 10.1101/mcs.a002584.

Biogenesis of respiratory cytochromes in bacteria.

Thony-Meyer L Microbiol Mol Biol Rev. 1997; 61(3):337-76.

PMID: 9293186 PMC: 232615. DOI: 10.1128/mmbr.61.3.337-376.1997.

Genes encoding the same three subunits of respiratory complex II are present in the mitochondrial DNA of two phylogenetically distant eukaryotes.

Burger G, Lang B, Reith M, Gray M Proc Natl Acad Sci U S A. 1996; 93(6):2328-32.

PMID: 8637872 PMC: 39795. DOI: 10.1073/pnas.93.6.2328.

The menaquinol oxidase of Bacillus subtilis W23.

Lemma E, Schagger H, Kroger A Arch Microbiol. 1993; 159(6):574-8.

PMID: 8394685 DOI: 10.1007/BF00249037.

Transcriptional regulation of a promoter in the men gene cluster of Bacillus subtilis.

Miller P, Mueller J, Hill K, Taber H J Bacteriol. 1988; 170(6):2742-8.

PMID: 3131310 PMC: 211197. DOI: 10.1128/jb.170.6.2742-2748.1988.

References

Capaldi R, Vanderkooi G . The low polarity of many membrane proteins. Proc Natl Acad Sci U S A. 1972; 69(4):930-2. PMC: 426597. DOI: 10.1073/pnas.69.4.930. View

Doi R . Genetic engineering in Bacillus subtilis. Biotechnol Genet Eng Rev. 1984; 2:121-55. DOI: 10.1080/02648725.1984.10647797. View

Ohne M . Regulation of the dicarboxylic acid part of the citric acid cycle in Bacillus subtilis. J Bacteriol. 1975; 122(1):224-34. PMC: 235661. DOI: 10.1128/jb.122.1.224-234.1975. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Kita K, Yamato I, Anraku Y . Purification and properties of cytochrome b556 in the respiratory chain of aerobically grown Escherichia coli K12. J Biol Chem. 1978; 253(24):8910-5. View

Hederstedt L, Holmgren E, Rutberg L . Characterization of a succinate dehydrogenase complex solubilized from the cytoplasmic membrane of Bacillus subtilis with the nonionic detergent Triton X-100. J Bacteriol. 1979; 138(2):370-6. PMC: 218187. DOI: 10.1128/jb.138.2.370-376.1979. View

Hederstedt L . Cytochrome b reducible by succinate in an isolated succinate dehydrogenase-cytochrome b complex from Bacillus subtilis membranes. J Bacteriol. 1980; 144(3):933-40. PMC: 294755. DOI: 10.1128/jb.144.3.933-940.1980. View

Messing J, Crea R, Seeburg P . A system for shotgun DNA sequencing. Nucleic Acids Res. 1981; 9(2):309-21. PMC: 326694. DOI: 10.1093/nar/9.2.309. View

Hederstedt L, Rutberg L . Succinate dehydrogenase--a comparative review. Microbiol Rev. 1981; 45(4):542-55. PMC: 281527. DOI: 10.1128/mr.45.4.542-555.1981. View

10.

KYTE J, Doolittle R . A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982; 157(1):105-32. DOI: 10.1016/0022-2836(82)90515-0. View

11.

Moran Jr C, Lang N, LeGrice S, Lee G, Stephens M, Sonenshein A . Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis. Mol Gen Genet. 1982; 186(3):339-46. DOI: 10.1007/BF00729452. View

12.

Hederstedt L, Rutberg L . Orientation of succinate dehydrogenase and cytochrome b558 in the Bacillus subtilis cytoplasmic membrane. J Bacteriol. 1983; 153(1):57-65. PMC: 217341. DOI: 10.1128/jb.153.1.57-65.1983. View

13.

Johnson W, Moran Jr C, Losick R . Two RNA polymerase sigma factors from Bacillus subtilis discriminate between overlapping promoters for a developmentally regulated gene. Nature. 1983; 302(5911):800-4. DOI: 10.1038/302800a0. View

14.

Magnusson K, RUTBERG B, Hederstedt L, Rutberg L . Characterization of a pleiotropic succinate dehydrogenase-negative mutant of Bacillus subtilis. J Gen Microbiol. 1983; 129(4):917-22. DOI: 10.1099/00221287-129-4-917. View

15.

Widger W, Cramer W, Herrmann R, Trebst A . Sequence homology and structural similarity between cytochrome b of mitochondrial complex III and the chloroplast b6-f complex: position of the cytochrome b hemes in the membrane. Proc Natl Acad Sci U S A. 1984; 81(3):674-8. PMC: 344897. DOI: 10.1073/pnas.81.3.674. View

16.

Kabsch W, Sander C . On the use of sequence homologies to predict protein structure: identical pentapeptides can have completely different conformations. Proc Natl Acad Sci U S A. 1984; 81(4):1075-8. PMC: 344767. DOI: 10.1073/pnas.81.4.1075. View

17.

Wong S, PRICE C, Goldfarb D, Doi R . The subtilisin E gene of Bacillus subtilis is transcribed from a sigma 37 promoter in vivo. Proc Natl Acad Sci U S A. 1984; 81(4):1184-8. PMC: 344790. DOI: 10.1073/pnas.81.4.1184. View

18.

Wood D, Darlison M, Wilde R, Guest J . Nucleotide sequence encoding the flavoprotein and hydrophobic subunits of the succinate dehydrogenase of Escherichia coli. Biochem J. 1984; 222(2):519-34. PMC: 1144207. DOI: 10.1042/bj2220519. View

19.

Murakami H, Kita K, Oya H, Anraku Y . Chromosomal location of the Escherichia coli cytochrome b556 gene, cybA. Mol Gen Genet. 1984; 196(1):1-5. DOI: 10.1007/BF00334084. View

20.

Murakami H, Kita K, Anraku Y . Cloning of cybB, the gene for cytochrome b561 of Escherichia coli K12. Mol Gen Genet. 1984; 198(2):1-6. DOI: 10.1007/BF00328692. View