Relevance of Oxygen Concentration in Stem Cell Culture for Regenerative Medicine

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2019 Mar 13

PMID 30857245

Citations 83

Authors

Cristina Mas-Bargues

Jorge Sanz-Ros

Aurora Roman-Dominguez

Marta Ingles

Lucia Gimeno-Mallench

Marya El Alami

Jose Vina-Almunia

Juan Gambini

Jose Vina

Consuelo Borras

Affiliations

Soon will be listed here.

Abstract

The key hallmark of stem cells is their ability to self-renew while keeping a differentiation potential. Intrinsic and extrinsic cell factors may contribute to a decline in these stem cell properties, and this is of the most importance when culturing them. One of these factors is oxygen concentration, which has been closely linked to the maintenance of stemness. The widely used environmental 21% O₂ concentration represents a hyperoxic non-physiological condition, which can impair stem cell behaviour by many mechanisms. The goal of this review is to understand these mechanisms underlying the oxygen signalling pathways and their negatively-associated consequences. This may provide a rationale for culturing stem cells under physiological oxygen concentration for stem cell therapy success, in the field of tissue engineering and regenerative medicine.

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