Glycosylation Profile of the Transferrin Receptor in Gestational Iron Deficiency and Early-Onset Severe Preeclampsia

Overview

Journal J Pregnancy

Publisher Wiley

Specialty Gynecology & Obstetrics

Date 2019 Mar 12

PMID 30854239

Citations 4

Authors

Alejandra Maria Gomez-Gutierrez

Beatriz Elena Parra-Sosa

Julio Cesar Bueno-Sanchez

Affiliations

Soon will be listed here.

Abstract

Objective: To examine the expression of hypoxia-inducible factor-1 (HIF-1), TfR1, and TfR1-attached terminal monosaccharides in placentas of women with IDAP and severe preeclampsia.

Methods: TfR1 and HIF-1 were detected by western blot. Immunoadsorption of TfR1 was performed to characterize the terminal monosaccharides by specific lectin binding.

Results: There was no difference in the expression of TfR1 and HIF-1 between groups. Lectin blot analysis pointed out an overexpression of galactose 1-4 -acetylglucosamine (Gal-GlcNAc) and mannose in severe preeclampsia.

Conclusion: The increase in Gal-GlcNAc may be due to the increased presence of antennary structures and the mannose glycans of TfR1 may indicate the presence of misfolded or incomplete proteins. These findings may be associated with the low expression of placental TfR1 in women with preeclampsia.

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