» Articles » PMID: 30847851

Enhancement of the Enzymatic Cellulose Saccharification by Penicillium Verruculosum Multienzyme Cocktails Containing Homologously Overexpressed Lytic Polysaccharide Monooxygenase

Overview
Journal Mol Biol Rep
Specialty Molecular Biology
Date 2019 Mar 9
PMID 30847851
Citations 4
Authors
Affiliations
Soon will be listed here.
Abstract

The gene lpmo1 encoding Penicillium verruculosum lytic polysaccharide monooxygenase (PvLPMO9A) was sequenced and homologously overexpressed in P. verruculosum B1-537 (ΔniaD) auxotrophic strain under the control of the cbh1 gene promoter in combination with either the cbh1 signal sequence (sCBH1-X series of samples) or the native lpmo1 signal sequence (sLPMO1-X series). Three enzyme samples of the sCBH1-X series were characterized by a lower overall content of cellobiohydrolases (CBHs: 26-45%) but slightly higher content of endoglucanases (EGs: 17-23%) relative to the reference B1-537 preparation (60% of CBHs and 14% of EGs), while the PvLPMO9A content in them made up 9-21% of the total secreted protein. The PvLPMO9A content in four enzyme preparations of the sLPMO1-X series was much higher (30-57%), however the portion of CBHs in most of them (except for sLPMO1-8) decreased even to a greater extent (to 21-42%) than in the samples of the sCBH1-X series. Two enzyme preparations (sCBH1-8 and sLPMO1-8), in which the content of cellulases was substantially retained and the portion of PvLPMO9A was 9-30%, demonstrated the increased yields of reducing sugars in 48-h saccharification of Avicel and milled aspen wood: 19-31 and 11-26%, respectively, compared to the reference cellulase cocktail.

Citing Articles

Genome and secretome insights: unravelling the lignocellulolytic potential of Myceliophthora verrucosa for enhanced hydrolysis of lignocellulosic biomass.

Sharma G, Kaur B, Singh V, Raheja Y, Di Falco M, Tsang A Arch Microbiol. 2024; 206(5):236.

PMID: 38676717 DOI: 10.1007/s00203-024-03974-w.


Interaction of carbohydrate binding module 20 with starch substrates.

Ngo S, Tran-Le P, Ho G, Le L, Bui L, Vu B RSC Adv. 2022; 9(43):24833-24842.

PMID: 35528656 PMC: 9069913. DOI: 10.1039/c9ra01981b.


Synergistic Action of a Lytic Polysaccharide Monooxygenase and a Cellobiohydrolase from in Cellulose Saccharification under High-Level Substrate Loading.

A Ogunyewo O, Randhawa A, Gupta M, Kaladhar V, Verma P, Yazdani S Appl Environ Microbiol. 2020; 86(23).

PMID: 32978122 PMC: 7657632. DOI: 10.1128/AEM.01769-20.


Insights into the cellulose degradation mechanism of the thermophilic fungus based on integrated functional omics.

Li X, Han C, Li W, Chen G, Wang L Biotechnol Biofuels. 2020; 13:143.

PMID: 32817759 PMC: 7425565. DOI: 10.1186/s13068-020-01783-z.

References
1.
Sinitsyna O, Bukhtoyarov F, Gusakov A, Okunev O, Bekkarevitch A, Vinetsky Y . Isolation and properties of major components of Penicillium canescens extracellular enzyme complex. Biochemistry (Mosc). 2003; 68(11):1200-9. DOI: 10.1023/b:biry.0000009134.48246.7e. View

2.
Markov A, Gusakov A, Kondratyeva E, Okunev O, Bekkarevich A, Sinitsyn A . New effective method for analysis of the component composition of enzyme complexes from Trichoderma reesei. Biochemistry (Mosc). 2005; 70(6):657-63. DOI: 10.1007/s10541-005-0166-4. View

3.
Morozova V, Gusakov A, Andrianov R, Pravilnikov A, Osipov D, Sinitsyn A . Cellulases of Penicillium verruculosum. Biotechnol J. 2010; 5(8):871-80. DOI: 10.1002/biot.201000050. View

4.
Vaaje-Kolstad G, Westereng B, Horn S, Liu Z, Zhai H, Sorlie M . An oxidative enzyme boosting the enzymatic conversion of recalcitrant polysaccharides. Science. 2010; 330(6001):219-22. DOI: 10.1126/science.1192231. View

5.
Phillips C, Beeson W, Cate J, Marletta M . Cellobiose dehydrogenase and a copper-dependent polysaccharide monooxygenase potentiate cellulose degradation by Neurospora crassa. ACS Chem Biol. 2011; 6(12):1399-406. DOI: 10.1021/cb200351y. View