Plant-produced Recombinant Osteopontin-Fc Fusion Protein Enhanced Osteogenesis

Overview

Journal Biotechnol Rep (Amst)

Specialty Biotechnology

Date 2019 Mar 9

PMID 30847284

Citations 11

Authors

Kaewta Rattanapisit

Suchada Srifa

Pornjira Kaewpungsup

Prasit Pavasant

Waranyoo Phoolcharoen

Affiliations

Soon will be listed here.

Abstract

Osteopontin (OPN) plays an important role in the bone regeneration process. Previous investigation showed that recombinant human OPN was able to express in leaves and induced the osteogenic related genes. Nevertheless, the purification of OPN from plant proteins with Ni affinity chromatography was still not effective enough. To improve the quality of protein expression and purification in plants, we constructed an Fc-based form of OPN. The complete OPN protein was fused to the human IgG1 Fc domain. Here, we showed that the plant-produced OPN-Fc increases the protein expression level and facilitates the purification of the recombinant protein. Our result showed that the plant-produced OPN-Fc can stimulate the expression of osteogenic related genes such as DMP1, OSX, and Wnt3a and also the calcium deposition in hPDL cells. These findings suggest that the plant-produced OPN-Fc has potential application in tissue engineering in the future.

Citing Articles

Unlocking the full potential of plant cell-based production for valuable proteins: Challenges and innovative strategies.

Xu J, PerezSanchez P, Sadravi S Biotechnol Adv. 2025; 79:108526.

PMID: 39914685 PMC: 11845290. DOI: 10.1016/j.biotechadv.2025.108526.

Chloroplast transformation for bioencapsulation and oral delivery using the immunoglobulin G fragment crystallizable (Fc) domain.

LaManna L, Chou C, Lei H, Barton E, Maliga P Sci Rep. 2023; 13(1):18916.

PMID: 37919321 PMC: 10622566. DOI: 10.1038/s41598-023-45698-9.

Development of HEK293T-produced recombinant receptor-Fc proteins as potential candidates against canine distemper virus.

Song L, Shan H, Huang J Front Vet Sci. 2023; 10:1180673.

PMID: 37215466 PMC: 10196245. DOI: 10.3389/fvets.2023.1180673.

Plant Molecular Pharming and Plant-Derived Compounds towards Generation of Vaccines and Therapeutics against Coronaviruses.

Venkataraman S Vaccines (Basel). 2022; 10(11).

PMID: 36366313 PMC: 9699630. DOI: 10.3390/vaccines10111805.

Improving Protein Quantity and Quality-The Next Level of Plant Molecular Farming.

Liu H, Timko M Int J Mol Sci. 2022; 23(3).

PMID: 35163249 PMC: 8836236. DOI: 10.3390/ijms23031326.

References

Lu Z, Lee K, Shao Y, Lee J, So Y, Choo Y . Expression of GA733-Fc fusion protein as a vaccine candidate for colorectal cancer in transgenic plants. J Biomed Biotechnol. 2012; 2012:364240. PMC: 3366255. DOI: 10.1155/2012/364240. View

Osathanon T, Ritprajak P, Nowwarote N, Manokawinchoke J, Giachelli C, Pavasant P . Surface-bound orientated Jagged-1 enhances osteogenic differentiation of human periodontal ligament-derived mesenchymal stem cells. J Biomed Mater Res A. 2012; 101(2):358-67. DOI: 10.1002/jbm.a.34332. View

Phoolcharoen W, Bhoo S, Lai H, Ma J, Arntzen C, Chen Q . Expression of an immunogenic Ebola immune complex in Nicotiana benthamiana. Plant Biotechnol J. 2011; 9(7):807-16. PMC: 4022790. DOI: 10.1111/j.1467-7652.2011.00593.x. View

Shapiro A, Ragni M, Valentino L, Key N, Josephson N, Powell J . Recombinant factor IX-Fc fusion protein (rFIXFc) demonstrates safety and prolonged activity in a phase 1/2a study in hemophilia B patients. Blood. 2011; 119(3):666-72. PMC: 3265197. DOI: 10.1182/blood-2011-07-367003. View

Knauf M, BELL D, Hirtzer P, Luo Z, Young J, Katre N . Relationship of effective molecular size to systemic clearance in rats of recombinant interleukin-2 chemically modified with water-soluble polymers. J Biol Chem. 1988; 263(29):15064-70. View

Cummings J, Guerrero M, Moon J, Waterman P, Nielsen R, Jefferson S . Safety and immunogenicity of a plant-produced recombinant monomer hemagglutinin-based influenza vaccine derived from influenza A (H1N1)pdm09 virus: a Phase 1 dose-escalation study in healthy adults. Vaccine. 2013; 32(19):2251-9. PMC: 9007152. DOI: 10.1016/j.vaccine.2013.10.017. View

Stadtfeld M, Brennand K, Hochedlinger K . Reprogramming of pancreatic beta cells into induced pluripotent stem cells. Curr Biol. 2008; 18(12):890-4. PMC: 2819222. DOI: 10.1016/j.cub.2008.05.010. View

Tuettenberg J, Seiz M, Debatin K, Hollburg W, VON Staden M, Thiemann M . Pharmacokinetics, pharmacodynamics, safety and tolerability of APG101, a CD95-Fc fusion protein, in healthy volunteers and two glioma patients. Int Immunopharmacol. 2012; 13(1):93-100. DOI: 10.1016/j.intimp.2012.03.004. View

Nillesen S, Geutjes P, Wismans R, Schalkwijk J, Daamen W, van Kuppevelt T . Increased angiogenesis and blood vessel maturation in acellular collagen-heparin scaffolds containing both FGF2 and VEGF. Biomaterials. 2006; 28(6):1123-31. DOI: 10.1016/j.biomaterials.2006.10.029. View

10.

Gan Y, Dang N, Qu Z, Shi R, Ding L, Wang L . GLP-1-Exendin-4/IgG4 (Fc) fusion protein as a novel drug for diabetes treatment. Exp Clin Endocrinol Diabetes. 2015; 123(6):371-5. DOI: 10.1055/s-0035-1548797. View

11.

Moreland L, Baumgartner S, Schiff M, Tindall E, Fleischmann R, Weaver A . Treatment of rheumatoid arthritis with a recombinant human tumor necrosis factor receptor (p75)-Fc fusion protein. N Engl J Med. 1997; 337(3):141-7. DOI: 10.1056/NEJM199707173370301. View

12.

Olinger Jr G, Pettitt J, Kim D, Working C, Bohorov O, Bratcher B . Delayed treatment of Ebola virus infection with plant-derived monoclonal antibodies provides protection in rhesus macaques. Proc Natl Acad Sci U S A. 2012; 109(44):18030-5. PMC: 3497800. DOI: 10.1073/pnas.1213709109. View

13.

McCaw T, Koepf E, Conley L . Evaluation of a novel methacrylate-based Protein A resin for the purification of immunoglobulins and Fc-fusion proteins. Biotechnol Prog. 2014; 30(5):1125-36. PMC: 4415579. DOI: 10.1002/btpr.1951. View

14.

Wang H, Davis J, Wu X . Immunoglobulin Fc domain fusion to TRAIL significantly prolongs its plasma half-life and enhances its antitumor activity. Mol Cancer Ther. 2014; 13(3):643-50. DOI: 10.1158/1535-7163.MCT-13-0645. View

15.

Zhao B, Zhang X, Krummenacher C, Song S, Gao L, Zhang H . Immunization With Fc-Based Recombinant Epstein-Barr Virus gp350 Elicits Potent Neutralizing Humoral Immune Response in a BALB/c Mice Model. Front Immunol. 2018; 9:932. PMC: 5938345. DOI: 10.3389/fimmu.2018.00932. View

16.

Kim M, Copland A, Nayak K, Chandele A, Ahmed M, Zhang Q . Plant-expressed Fc-fusion protein tetravalent dengue vaccine with inherent adjuvant properties. Plant Biotechnol J. 2017; 16(7):1283-1294. PMC: 5999314. DOI: 10.1111/pbi.12869. View

17.

Shrivats A, McDermott M, Hollinger J . Bone tissue engineering: state of the union. Drug Discov Today. 2014; 19(6):781-6. DOI: 10.1016/j.drudis.2014.04.010. View

18.

Lee S, Shin H . Matrices and scaffolds for delivery of bioactive molecules in bone and cartilage tissue engineering. Adv Drug Deliv Rev. 2007; 59(4-5):339-59. DOI: 10.1016/j.addr.2007.03.016. View

19.

Huang Z, Phoolcharoen W, Lai H, Piensook K, Cardineau G, Zeitlin L . High-level rapid production of full-size monoclonal antibodies in plants by a single-vector DNA replicon system. Biotechnol Bioeng. 2010; 106(1):9-17. PMC: 2905544. DOI: 10.1002/bit.22652. View

20.

Sockolosky J, Kivimae S, Szoka F . Fusion of a short peptide that binds immunoglobulin G to a recombinant protein substantially increases its plasma half-life in mice. PLoS One. 2014; 9(7):e102566. PMC: 4109916. DOI: 10.1371/journal.pone.0102566. View