A SIR-independent Role for Cohesin in Subtelomeric Silencing and Organization

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2019 Mar 8

PMID 30842278

Citations 7

Authors

Deepash Kothiwal

Shikha Laloraya

Affiliations

Soon will be listed here.

Abstract

Cohesin is a key determinant of chromosome architecture due to its DNA binding and tethering ability. Cohesin binds near centromeres and chromosome arms and also close to telomeres, but its role near telomeres remains elusive. In budding yeast, transcription within 20 kb of telomeres is repressed, in part by the histone-modifying silent information regulator (SIR) complex. However, extensive subtelomeric repressed domains lie outside the SIR-binding region, but the mechanism of silencing in these regions remains poorly understood. Here, we report a role for cohesin in subtelomeric silencing that extends even beyond the zone of SIR binding. Clusters of subtelomeric genes were preferentially derepressed in a cohesin mutant, whereas SIR binding was unaltered. Genetic interactions with known telomere silencing factors indicate that cohesin operates independent of the SIR-mediated pathway for telomeric silencing. Mutant cells exhibited Mpk1-dependent Sir3 hyperphosphorylation that contributes to subtelomeric derepression to a limited extent. Compaction of subtelomeric domains and tethering to the nuclear envelope were impaired in mutant cells. Our findings provide evidence for a unique SIR-independent mechanism of subtelomeric repression mediated by cohesin.

Citing Articles

Cohesin contributes to transcriptional repression of stage-specific genes in the human malaria parasite.

Rosa C, Singh P, Chen P, Sinha A, Claes A, Preiser P EMBO Rep. 2023; 24(10):e57090.

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ISW1a modulates cohesin distribution in centromeric and pericentromeric regions.

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Substrates of the MAPK Slt2: Shaping Yeast Cell Integrity.

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