LINC00702 Suppresses Proliferation and Invasion in Non-small Cell Lung Cancer Through Regulating MiR-510/PTEN Axis

Overview

Journal Aging (Albany NY)

Specialty Geriatrics

Date 2019 Mar 7

PMID 30840927

Citations 23

Authors

Wencheng Yu

Daowei Li

Xiaoyan Ding

Yong Sun

Yanli Liu

Jinpeng Cong

Jiong Yang

Jian Sun

Xuchao Ning

Hongmei Wang

Tao Xu

Affiliations

Soon will be listed here.

Abstract

Background: Long non-coding RNAs (lncRNAs) have been consistently reported to be involved in the progression of non-small cell lung cancer (NSCLC). In this study, we aimed to identify aberrantly expressed lncRNAs in NSCLC, in order to explore new therapeutic targets for NSCLC.

Methods: Two pairs of NSCLC and adjacent normal tissues were first analyzed by RNA sequencing. The expressions of LINC00702 in 40 pairs patient samples and in 4 NSCLC cell lines was measured by quantitative real-time PCR. Putative target miRNAs of LINC00702 were predicted by the bioinformatics tools. The effect of LINC00702 on tumor growth was evaluated.

Results: LINC00702 was significantly down-regulated in patients with NSCLC, which was correlated with tumor size and metastasis. In addition, overexpression of LINC00702 markedly suppressed proliferation and metastasis in NSCLC cells via inducing apoptosis and . Moreover, bioinformatics and luciferase reporter assays demonstrated that LINC00702 functioned as a competing endogenous RNA (ceRNA) for miR-510 in NSCLC, and upregulated its target gene PTEN.

Conclusion: Our results indicated that LINC00702 modulated the expression of PTEN gene by acting as a ceRNA for miR-510 in NSCLC. Therefore, LINC00702 may serve as a potential target for the diagnosis and treatment of patients with NSCLC.

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