Multi-Epitope Fusion Protein Eg MefAg-1 As a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep

Overview

Journal Korean J Parasitol

Specialty Parasitology

Date 2019 Mar 7

PMID 30840802

Citations 1

Authors

Liu Tianli

Wang Xifeng

Tian Zhenzhong

Wang Lixia

Zhang Xingxing

Qiao Jun

Meng Qingling

Gong Shasha

Chen Ying

Cai Xuepeng

Affiliations

Soon will be listed here.

Abstract

Cystic echinococcosis (CE) in sheep is a hazardous zoonotic parasitic disease that is caused by Echinococcus granulosus (Eg). At present, serological test is an important diagnostic method for Eg infection in domestic animals. Here, a fusion protein Eg mefAg-1 harboring 8 dominant B-cell epitopes of Eg such as antigen B, tetraspanin 1, tetraspanin 6, reticulon and Eg95 was produced in E. coli and evaluated for CE in sheep by indirect ELISA. Eg mefAg-1 showed in ELISA a high sensitivity (93.41%) and specificity (99.31%), with a coincidence rate of 97.02%. Overall, it is suggested that the Eg mefAg-1 could be a potential antigen candidate for CE serodiagnosis in sheep.

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