Single Capillary Oximetry and Tissue Ultrastructural Sensing by Dual-band Dual-scan Inverse Spectroscopic Optical Coherence Tomography

Overview

Journal Light Sci Appl

Publisher Springer Nature

Date 2019 Mar 7

PMID 30839641

Citations 16

Authors

Rongrong Liu

James A Winkelmann

Graham Spicer

Yunxiao Zhu

Aya Eid

Guillermo A Ameer

Vadim Backman

Ji Yi

Affiliations

Soon will be listed here.

Abstract

Measuring capillary oxygenation and the surrounding ultrastructure can allow one to monitor a microvascular niche and better understand crucial biological mechanisms. However, capillary oximetry and pericapillary ultrastructure are challenging to measure in vivo. Here we demonstrate a novel optical imaging system, dual-band dual-scan inverse spectroscopic optical coherence tomography (D2-ISOCT), that, for the first time, can simultaneously obtain the following metrics in vivo using endogenous contrast: (1) capillary-level oxygen saturation and arteriolar-level blood flow rates, oxygen delivery rates, and oxygen metabolic rates; (2) spatial characteristics of tissue structures at length scales down to 30 nm; and (3) morphological images up to 2 mm in depth. To illustrate the capabilities of D2-ISOCT, we monitored alterations to capillaries and the surrounding pericapillary tissue (tissue between the capillaries) in the healing response of a mouse ear wound model. The obtained microvascular and ultrastructural metrics corroborated well with each other, showing the promise of D2-ISOCT for becoming a powerful new non-invasive imaging tool.

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