Characterization of Collagen Fibers (I, III, IV) and Elastin of Normal and Neoplastic Canine Prostatic Tissues

Overview

Journal Vet Sci

Publisher MDPI

Specialty Veterinary Medicine

Date 2019 Mar 6

PMID 30832371

Citations 4

Authors

Luis Gabriel Rivera Calderon

Priscila Emiko Kobayashi

Rosemeri Oliveira Vasconcelos

Carlos Eduardo Fonseca-Alves

Renee Laufer-Amorim

Affiliations

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Abstract

This study aimed to investigate collagen (Coll-I, III, IV) and elastin in canine normal prostate and prostate cancer (PC) using Picrosirius red (PSR) and Immunohistochemical (IHC) analysis. Eight normal prostates and 10 PC from formalin-fixed, paraffin-embedded samples were used. Collagen fibers area was analyzed with ImageJ software. The distribution of Coll-I and Coll-III was approximately 80% around prostatic ducts and acini, 15% among smooth muscle, and 5% surrounding blood vessels, in both normal prostate and PC. There was a higher median area of Coll-III in PC when compared to normal prostatic tissue ( = 0.001 for PSR and = 0.05 for IHC). Immunostaining for Coll-IV was observed in the basal membrane of prostate acini, smooth muscle, blood vessels, and nerve fibers of normal and PC samples. Although there was no difference in Coll-IV area between normal tissue and PC, tumors with Gleason score 10 showed absence of Coll-IV, when compared to scores 6 and 8 ( = 0.0095). Elastic fibers were found in the septa dividing the lobules and around the prostatic acini of normal samples and were statistically higher in PC compared to normal tissue ( = 0.00229). Investigation of ECM components brings new information and should be correlated with prognosis in future studies.

Citing Articles

Comparative Pathobiology of Canine and Human Prostate Cancer: State of the Art and Future Directions.

Nascente E, Amorim R, Fonseca-Alves C, de Moura V Cancers (Basel). 2022; 14(11).

PMID: 35681707 PMC: 9179314. DOI: 10.3390/cancers14112727.

Visualization and Quantification of the Extracellular Matrix in Prostate Cancer Using an Elastin Specific Molecular Probe.

Kader A, Brangsch J, Reimann C, Kaufmann J, Mangarova D, Moeckel J Biology (Basel). 2021; 10(11).

PMID: 34827210 PMC: 8615039. DOI: 10.3390/biology10111217.

An RNA-Seq-Based Framework for Characterizing Canine Prostate Cancer and Prioritizing Clinically Relevant Biomarker Candidate Genes.

Thiemeyer H, Taher L, Schille J, Packeiser E, Harder L, Hewicker-Trautwein M Int J Mol Sci. 2021; 22(21).

PMID: 34768937 PMC: 8584104. DOI: 10.3390/ijms222111481.

A Comparative in Silico Analysis of CD24's Prognostic Value in Human and Canine Prostate Cancer.

Leis-Filho A, de Faria Lainetti P, Simao Franzoni M, Palmieri C, Kobayshi P, Laufer-Amorim R J Pers Med. 2021; 11(3).

PMID: 33806857 PMC: 8004660. DOI: 10.3390/jpm11030232.

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