Ion Chromatographic Fingerprinting of STC-1 Cellular Response for Taste Sensing

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2019 Mar 6

PMID 30832321

Authors

Marcin Zabadaj

Aleksandra Szuplewska

Maria Balcerzak

Michal Chudy

Patrycja Ciosek-Skibinska

Affiliations

Soon will be listed here.

Abstract

Taste sensing is of great importance in both the pharmaceutical and foodstuff industries, and is currently mainly based on human sensory evaluation. Many approaches based on chemical sensors have been proposed, leading to the development of various electronic tongue systems. However, this approach is limited by the applied recognition methods, which do not consider natural receptors. Biorecognition elements such as taste receptor proteins or whole cells can be involved in the development of taste sensing biosensors usually equipped with various electrochemical transducers. Here, we propose a new approach: intestinal secretin tumor cell line (STC-1) chemosensory cells were applied for taste recognition, and their taste-specific cellular response was decoded from ion chromatographic fingerprints with the use of multivariate data processing by partial least squares discriminant analysis (PLS-DA). This approach could be useful for the development of various non-invasive taste sensing assays, as well as for studying taste transduction mechanisms in vitro.

References

Chen M, Wu S, Reeve Jr J, Rozengurt E . Bitter stimuli induce Ca2+ signaling and CCK release in enteroendocrine STC-1 cells: role of L-type voltage-sensitive Ca2+ channels. Am J Physiol Cell Physiol. 2006; 291(4):C726-39. DOI: 10.1152/ajpcell.00003.2006. View

Ciosek P, Wroblewski W . Sensor arrays for liquid sensing--electronic tongue systems. Analyst. 2007; 132(10):963-78. DOI: 10.1039/b705107g. View

Wang T, Hui G, Deng S . A novel sweet taste cell-based sensor. Biosens Bioelectron. 2010; 26(2):929-34. DOI: 10.1016/j.bios.2010.06.049. View

Chaudhari N, Roper S . The cell biology of taste. J Cell Biol. 2010; 190(3):285-96. PMC: 2922655. DOI: 10.1083/jcb.201003144. View

Stockert J, Blazquez-Castro A, Canete M, Horobin R, Villanueva A . MTT assay for cell viability: Intracellular localization of the formazan product is in lipid droplets. Acta Histochem. 2012; 114(8):785-96. DOI: 10.1016/j.acthis.2012.01.006. View

Hui G, Mi S, Deng S . Sweet and bitter tastants specific detection by the taste cell-based sensor. Biosens Bioelectron. 2012; 35(1):429-438. DOI: 10.1016/j.bios.2012.02.028. View

Barham H, Cooper S, Anderson C, Tizzano M, Kingdom T, Finger T . Solitary chemosensory cells and bitter taste receptor signaling in human sinonasal mucosa. Int Forum Allergy Rhinol. 2013; 3(6):450-7. PMC: 3655139. DOI: 10.1002/alr.21149. View

Wu C, Du L, Zou L, Huang L, Wang P . A biomimetic bitter receptor-based biosensor with high efficiency immobilization and purification using self-assembled aptamers. Analyst. 2013; 138(20):5989-94. DOI: 10.1039/c3an01291c. View

Du L, Zou L, Zhao L, Huang L, Wang P, Wu C . Label-free functional assays of chemical receptors using a bioengineered cell-based biosensor with localized extracellular acidification measurement. Biosens Bioelectron. 2013; 54:623-7. DOI: 10.1016/j.bios.2013.11.049. View

10.

Song H, Jin H, Ahn S, Kim D, Lee S, Kim U . Bioelectronic tongue using heterodimeric human taste receptor for the discrimination of sweeteners with human-like performance. ACS Nano. 2014; 8(10):9781-9. DOI: 10.1021/nn502926x. View

11.

Du L, Zou L, Zhao L, Wang P, Wu C . Biomimetic chemical sensors using bioengineered olfactory and taste cells. Bioengineered. 2014; 5(5):326-30. PMC: 4156494. DOI: 10.4161/bioe.29762. View

12.

Wu C, Lillehoj P, Wang P . Bioanalytical and chemical sensors using living taste, olfactory, and neural cells and tissues: a short review. Analyst. 2015; 140(21):7048-61. DOI: 10.1039/c5an01288k. View

13.

Ahn S, An J, Song H, Park J, Lee S, Kim J . Duplex Bioelectronic Tongue for Sensing Umami and Sweet Tastes Based on Human Taste Receptor Nanovesicles. ACS Nano. 2016; 10(8):7287-96. DOI: 10.1021/acsnano.6b02547. View

14.

Stone R, Stojadinovic O, Rosa A, Ramirez H, Badiavas E, Blumenberg M . A bioengineered living cell construct activates an acute wound healing response in venous leg ulcers. Sci Transl Med. 2017; 9(371). PMC: 5472448. DOI: 10.1126/scitranslmed.aaf8611. View

15.

Lee J, Cho A, Jin Y, Kim J, Kim S, Cho S . Bio-artificial tongue with tongue extracellular matrix and primary taste cells. Biomaterials. 2017; 151:24-37. DOI: 10.1016/j.biomaterials.2017.10.019. View

16.

Wu C, Du Y, Huang L, Ben-Shoshan Galeczki Y, Dagan-Wiener A, Naim M . Biomimetic Sensors for the Senses: Towards Better Understanding of Taste and Odor Sensation. Sensors (Basel). 2017; 17(12). PMC: 5750803. DOI: 10.3390/s17122881. View

17.

Son M, Park T . The bioelectronic nose and tongue using olfactory and taste receptors: Analytical tools for food quality and safety assessment. Biotechnol Adv. 2018; 36(2):371-379. DOI: 10.1016/j.biotechadv.2017.12.017. View

18.

Yue X, Liang J, Gu F, Du D, Chen F . Berberine activates bitter taste responses of enteroendocrine STC-1 cells. Mol Cell Biochem. 2018; 447(1-2):21-32. DOI: 10.1007/s11010-018-3290-3. View

19.

Ahn S, An J, Jang I, Na W, Yang H, Cho K . High-performance bioelectronic tongue using ligand binding domain T1R1 VFT for umami taste detection. Biosens Bioelectron. 2018; 117:628-636. DOI: 10.1016/j.bios.2018.06.028. View