Altered Arachidonic Acid Content in Polymorphonuclear and Mononuclear Cells from Patients with Allergic Rhinitis And/or Asthma

Overview

Journal Lipids

Specialty Biochemistry

Date 1986 Jan 1

PMID 3083171

Citations 8

Authors

R E Rocklin

L Thistle

L Gallant

M S Manku

D Horrobin

Affiliations

Soon will be listed here.

Abstract

We previously have found that monocytes from patients with allergic rhinitis and/or asthma produce less PGE2 than cells from normal subjects in response to a histamine-induced lymphokine. In order to investigate this observation further, we measured the fatty acid content in the total phospholipids derived from the plasma, red cells, buffy coat cells, neutrophils, monocytes and lymphocytes of 27 allergic patients and 21 normal controls. There were no substantial differences between atopics and normals in the fatty acid analyses carried out for plasma and red cells. However, linoleic acid (18:2n-6) levels were elevated significantly in the buffy coat fraction, while arachidonic acid (20:4n-6) levels were reduced. Measurement of fatty acid levels after fractionation of the buffy coat population into neutrophils and monocytes yielded similar elevations in 18:2n-6 and reduced 20:4n-6. In contrast, lymphocytes appeared to have the reverse pattern, i.e., significantly reduced 18:2n-6 and elevated 20:4n-6 levels. These data suggest that atopic leukocytes may have altered essential fatty acid metabolism.

Citing Articles

Association of Serum Polyunsaturated Fatty Acids (PUFAs) and Children's Dietary Inflammatory Index (C-DII) with Recurrent Respiratory Infections in Children: A Cross-Sectional Study.

Gorczyca D, Szeremeta K, Pasciak M, Szponar B, Zhao L, Wirth M Nutrients. 2025; 17(1.

PMID: 39796587 PMC: 11723345. DOI: 10.3390/nu17010153.

Maternal plasma phosphatidylcholine fatty acids and atopy and wheeze in the offspring at age of 6 years.

Pike K, Calder P, Inskip H, Robinson S, Roberts G, Cooper C Clin Dev Immunol. 2012; 2012:474613.

PMID: 23049600 PMC: 3463812. DOI: 10.1155/2012/474613.

Umbilical cord PUFA are determined by maternal and child fatty acid desaturase (FADS) genetic variants in the Avon Longitudinal Study of Parents and Children (ALSPAC).

Lattka E, Koletzko B, Zeilinger S, Hibbeln J, Klopp N, Ring S Br J Nutr. 2012; 109(7):1196-210.

PMID: 22877655 PMC: 3600399. DOI: 10.1017/S0007114512003108.

The relation between dietary intake of individual fatty acids, FEV1 and respiratory disease in Dutch adults.

McKeever T, Lewis S, Cassano P, Ocke M, Burney P, Britton J Thorax. 2007; 63(3):208-14.

PMID: 17901161 PMC: 3979330. DOI: 10.1136/thx.2007.090399.

Erythrocyte and plasma fatty acid patterns in dogs with atopic dermatitis and healthy dogs in the same household.

Fuhrmann H, Zimmermann A, Guck T, Oechtering G Can J Vet Res. 2006; 70(3):191-6.

PMID: 16850941 PMC: 1477930.

References

Manku M, Horrobin D, Huang Y, Morse N . Fatty acids in plasma and red cell membranes in normal humans. Lipids. 1983; 18(12):906-8. DOI: 10.1007/BF02534572. View

DODGE J, Phillips G . Composition of phospholipids and of phospholipid fatty acids and aldehydes in human red cells. J Lipid Res. 1967; 8(6):667-75. View

Giddings J, Piovella F, Ricetti M, Jarvis A, Peake I, Bloom A . Characterization of procoagulant activity produced by cultures of human monocytes and lymphocytes separated in colloidal silica-polvinylpyrrolidone gradients. Clin Lab Haematol. 1980; 2(2):121-8. DOI: 10.1111/j.1365-2257.1980.tb00815.x. View

Beer D, Dinarello C, Rosenwasser L, Rocklin R . Human monocyte-derived soluble product(s) has an accessory function in the generation of histamine- and concanavalin A-induced suppressor T cells. J Clin Invest. 1982; 70(2):393-400. PMC: 371248. DOI: 10.1172/jci110629. View

Beer D, Osband M, McCaffrey R, Soter N, Rocklin R . Abnormal histamine-induced suppressor-cell function in atopic subjects. N Engl J Med. 1982; 306(8):454-8. DOI: 10.1056/NEJM198202253060804. View

Willis A, Hassam A, Crawford M, Stevens P, Denton J . Relationships between prostaglandins, prostacyclin and EFA precursors in rabbits maintained on EFA-deficient diets. Prog Lipid Res. 1981; 20:161-7. DOI: 10.1016/0163-7827(81)90030-8. View

Beer D, Rosenwasser L, Dinarello C, Rocklin R . Cellular interactions in the generation and expression of histamine-induced suppressor activity. Cell Immunol. 1982; 69(1):101-12. DOI: 10.1016/0008-8749(82)90054-5. View

Rocklin R, Kiselis I, Beer D, Rossi P, Maggi F, Bellanti J . Augmentation of prostaglandin and thromboxane production in vitro by monocytes exposed to histamine-induced suppressor factor (HSF). Cell Immunol. 1983; 77(1):92-8. DOI: 10.1016/0008-8749(83)90009-6. View

Matloff S, Kiselis I, Rocklin R . Reduced production of histamine-induced suppressor factor (HSF) by atopic mononuclear cells and decreased prostaglandin E2 output by HSF-stimulated atopic monocytes. J Allergy Clin Immunol. 1983; 72(4):359-64. DOI: 10.1016/0091-6749(83)90500-6. View

10.

Manku M, Horrobin D, Morse N, Kyte V, Jenkins K, Wright S . Reduced levels of prostaglandin precursors in the blood of atopic patients: defective delta-6-desaturase function as a biochemical basis for atopy. Prostaglandins Leukot Med. 1982; 9(6):615-28. DOI: 10.1016/0262-1746(82)90019-1. View

11.

Huang Y, Mitchell J, Jenkins K, Manku M, Horrobin D . Effect of dietary depletion and repletion of linoleic acid on renal fatty acid composition and urinary prostaglandin excretion. Prostaglandins Leukot Med. 1984; 15(2):223-8. DOI: 10.1016/0262-1746(84)90179-3. View

12.

Wright S, Burton J . Oral evening-primrose-seed oil improves atopic eczema. Lancet. 1982; 2(8308):1120-2. DOI: 10.1016/s0140-6736(82)92784-2. View

13.

Beer D, Matloff S, Rocklin R . The influence of histamine on immune and inflammatory responses. Adv Immunol. 1984; 35:209-68. DOI: 10.1016/s0065-2776(08)60577-5. View