Children Attending Day Care Centers Are a Year-round Reservoir of Gastrointestinal Viruses

Overview

Journal Sci Rep

Specialty Science

Date 2019 Mar 3

PMID 30824842

Citations 20

Authors

Betina Hebbelstrup Jensen

Pikka Jokelainen

Alex Christian Yde Nielsen

Kristina Traeholt Franck

Dorthe Rejkjaer Holm

Kristian Schonning

Andreas M Petersen

Karen A Krogfelt

Affiliations

Soon will be listed here.

Abstract

Viral gastroenteritis causes high morbidity worldwide. In this study, stool samples from 179 children aged 0-6 years attending Danish day care centers were investigated for gastrointestinal viruses. Each child was observed for one year with submission of samples and questionnaires every two months. Adenovirus, norovirus, rotavirus, and sapovirus were detected in samples using real-time PCR. A total of 229 (33%) of the 688 samples collected tested positive for at least one virus. At the first sampling point, adenovirus was shed by 6%, norovirus genotype I by 3% and genotype II by 12%, rotavirus A by 9%, and sapovirus by 21% of the 142 children included in the risk factor analyses. Increasing age was identified as a protective factor against testing positive for gastrointestinal virus, whereas nausea during the previous two months was positively associated with testing positive. Odds of shedding adenovirus were 9.6 times higher among children treated with antibiotics within the previous two months than among children who were not. Gastrointestinal viruses were shed year-round and high viral loads were observed in samples from both symptomatic and asymptomatic children, suggesting children in day care as a reservoir and a possible source of spreading of viruses into the community.

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